To monitor the distribution of lymphocyte sub populations infiltrating tumors, a simplified modification of standard techniques was developed to identify the complement receptor B lymphocytes in frozen tissue sections. This technique is based on binding of antibody coated bovine erythrocytes by B lymphocytes in the presence of sub hemolytic concentrations of mouse complement. B lymphocytes can be identified in frozen tissue sections by adherent sensitized bovine erythrocytes. The specificity of the technique for B lymphocytes was established by absence of spontaneous rosette formation with bovine erythrocytes by human T lymphocytes, specific binding of bovine erythrocyte antibody complement complexes to B cell regions of normal lymph nodes and spleen and binding of erythrocyte antibody complement to a high proportion of chronic lymphocytic leukemia (a B cell leukemia) lymphocytes. Five transitional carcinomas studied had regions of mononuclear infiltration that were virtually devoid of complement receptor lymphocytes. The results suggest that lymphocytes infiltrating bladder carcinomas may be predominantly T lymphocytes.
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