Identification of complement receptor lymphocytes (B cells) in lymph nodes and tumor infiltrates

W. J. Catalona, R. Mann, F. Nime, C. Potvin, J. I. Harty, D. Gomolka, J. C. Eggleston

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

To monitor the distribution of lymphocyte sub populations infiltrating tumors, a simplified modification of standard techniques was developed to identify the complement receptor B lymphocytes in frozen tissue sections. This technique is based on binding of antibody coated bovine erythrocytes by B lymphocytes in the presence of sub hemolytic concentrations of mouse complement. B lymphocytes can be identified in frozen tissue sections by adherent sensitized bovine erythrocytes. The specificity of the technique for B lymphocytes was established by absence of spontaneous rosette formation with bovine erythrocytes by human T lymphocytes, specific binding of bovine erythrocyte antibody complement complexes to B cell regions of normal lymph nodes and spleen and binding of erythrocyte antibody complement to a high proportion of chronic lymphocytic leukemia (a B cell leukemia) lymphocytes. Five transitional carcinomas studied had regions of mononuclear infiltration that were virtually devoid of complement receptor lymphocytes. The results suggest that lymphocytes infiltrating bladder carcinomas may be predominantly T lymphocytes.

Original languageEnglish (US)
Pages (from-to)915-921
Number of pages7
JournalUnknown Journal
Volume114
Issue number6
DOIs
StatePublished - Jan 1 1975

ASJC Scopus subject areas

  • Urology

Fingerprint Dive into the research topics of 'Identification of complement receptor lymphocytes (B cells) in lymph nodes and tumor infiltrates'. Together they form a unique fingerprint.

Cite this