Identification of derlin-1 as a novel growth factor-responsive endothelial antigen by suppression subtractive hybridization

Yuliang Ran, Yangfu Jiang, Xing Zhong, Zhuan Zhou, Haiyan Liu, Hai Hu, Jin Ning Lou, Zhihua Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Endothelial cells play an important regulatory role in embryonic development, reproductive functions, tumor growth and progression. In the present study, the suppression subtractive hybridization (SSH) method was employed to identify differentially expressed genes between non-stimulated endothelial cells and activated endothelial cells. Following mRNA isolation of non-stimulated and hepatocellular carcinoma homogenate-stimulated cells, cDNAs of both populations were prepared and subtracted by suppressive PCR. Sequencing of the enriched cDNAs identified a couple of genes differentially expressed, including derlin-1. Derlin-1 was significantly up-regulated by tumor homogenates, VEGF, and endothelial growth supplements in a dose-dependent manner. Knock-down of derlin-1 triggered endothelial cell apoptosis, inhibited endothelial cell proliferation, and blocked the formation of a network of tubular-like structures. Our data reveal that derlin-1 is a novel growth factor-responsive endothelial antigen that promotes endothelial cell survival and growth.

Original languageEnglish (US)
Pages (from-to)1272-1278
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume348
Issue number4
DOIs
StatePublished - Oct 6 2006

Keywords

  • Angiogenesis
  • Cancer
  • Derlin-1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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