Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells

Dong mei Wang; L. Yang; Liang Li; Kai Feng; Ci xian Bai; Lin Chen; Xue tao Pei

Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells

Dong mei Wang^*, L. Yang, Liang Li, Kai Feng, Ci xian Bai, Lin Chen, Xue tao Pei

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

OBJECTIVE: To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells. METHODS: With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank. RESULTS: 593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences. CONCLUSION: Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.

Original language	English (US)
Pages (from-to)	423-425
Number of pages	3
Journal	Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
Volume	24
Issue number	8
State	Published - Aug 2003
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Cite this

@article{e1ed299f06074b0fbc64696bf0db07bb,

title = "Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells",

abstract = "OBJECTIVE: To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells. METHODS: With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank. RESULTS: 593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences. CONCLUSION: Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.",

author = "Wang, {Dong mei} and L. Yang and Liang Li and Kai Feng and Bai, {Ci xian} and Lin Chen and Pei, {Xue tao}",

note = "Copyright: This record is sourced from MEDLINE{\textregistered}/PubMed{\textregistered}, a database of the U.S. National Library of Medicine",

year = "2003",

month = aug,

language = "English (US)",

volume = "24",

pages = "423--425",

journal = "Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi",

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publisher = "Zhongguo Yixue Kexueyuan",

number = "8",

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TY - JOUR

T1 - Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells

AU - Wang, Dong mei

AU - Yang, L.

AU - Li, Liang

AU - Feng, Kai

AU - Bai, Ci xian

AU - Chen, Lin

AU - Pei, Xue tao

N1 - Copyright: This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

PY - 2003/8

Y1 - 2003/8

N2 - OBJECTIVE: To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells. METHODS: With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank. RESULTS: 593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences. CONCLUSION: Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.

AB - OBJECTIVE: To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells. METHODS: With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank. RESULTS: 593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences. CONCLUSION: Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.

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M3 - Article

C2 - 14642181

AN - SCOPUS:34447620998

SN - 0253-2727

VL - 24

SP - 423

EP - 425

JO - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

JF - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

IS - 8

ER -

Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells

Abstract

ASJC Scopus subject areas

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