OBJECTIVE: To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells. METHODS: With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank. RESULTS: 593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences. CONCLUSION: Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.
|Original language||English (US)|
|Number of pages||3|
|Journal||Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi|
|State||Published - Aug 2003|
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