Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells

Dong mei Wang*, L. Yang, Liang Li, Kai Feng, Ci xian Bai, Lin Chen, Xue tao Pei

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

OBJECTIVE: To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells. METHODS: With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank. RESULTS: 593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences. CONCLUSION: Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.

Original languageEnglish (US)
Pages (from-to)423-425
Number of pages3
JournalZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
Volume24
Issue number8
StatePublished - Aug 2003
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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