Identification of fatigue biomarkers in treated and treatment-naive HIV patients: Preliminary Results

Kelly Jensen*, Young Ah Goo, Anella Yahiaoui, Sundeep Bajwa, Dave Goodlett, Jim Russo, Joachim Voss

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Objective assessment in human immunodeficiency virus (HIV)-related fatigue has been elusive because the biological mechanisms are not well characterized. We tried to identify low-abundance plasma proteins that correlate with self-reported fatigue intensity in people living with HIV. We used plasma samples from 32 patients with HIV with varying degrees of fatigue who were either treated with nucleoside reverse transcriptase inhibitors or treatment näi{dotless}ve. The plasma samples were enriched for lowabundance proteins and trypsinized. The peptides were analyzed using shotgun proteomics. Five targets correlated with severity of fatigue: apolipoprotein A-1 (ApoA1), apolipoprotein B (ApoB), histine-rich glycoprotein, alpha-1 B glycoprotein, and orosomucoid 2. These targets were selected based on total abundance and spectral count differences, and ApoA1 and ApoB were analyzed via Western blots to verify the mass spectrometry results. ApoA1 levels were higher in untreated patients, while ApoB results suggested a possible positive trend in treated patients. Further analysis is needed to identify additional low-abundance proteins and confirm already-identified proteins as potential fatigue biomarkers.

Original languageEnglish (US)
Pages (from-to)278-287
Number of pages10
JournalBiological research for nursing
Volume16
Issue number3
DOIs
StatePublished - Jul 2014

Keywords

  • Antibodies
  • Biomarkers
  • Fatigue
  • HIV
  • Mitochondria
  • Proteins

ASJC Scopus subject areas

  • Research and Theory

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