Identification of genes regulated by UV/salicylic acid

T. Paunesku, C. M. Chang-Liu, P. Shearin-Jones, C. Watson, J. Milton, J. Oryhon, D. Salbego, A. Milosavljevic, G. E. Woloschak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Purpose: Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-κB. In the experiments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and without the addition of salicylic acid. Materials and methods: Differential-display RT-PCR was used to identify differentially expressed genes. Results: Eight such cDNAs are presented: lactate dehydrogenase (LDH-β), nuclear encoded mitochondrial NADH ubiquinone reductase 24 kDa (NDUFV2), elongation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear encoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA187906 and AA513156). Conclusions: Sequences of four of these genes contained NF-κB DNA binding sites of the type that may attract transrepressor p55/p55 NF-κB homodimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalInternational Journal of Radiation Biology
Volume76
Issue number2
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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