Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities

Y. Wan; M. W. Kirschner

doi:10.1073/pnas.231487598

Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities

Y. Wan, M. W. Kirschner^*

^*Corresponding author for this work

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

CDC20/CDH1 activates the anaphase-promoting complex (APC) and targets various substrates for degradation, thereby allowing the ordered progression through mitosis and G₁. We have found multiple functional CDH1 homologues in the chick. The transcripts of these novel genes are differentially localized to proliferating, differentiated, and postmitotic tissues. All four proteins bind and form a complex with APC in vitro and in cultural cells and have quantitatively different activities in mediating ubiquitination of various APC substrates. Our results suggest that multiple CDH1s may temporally and spatially regulate APC activity both within and outside of the cell cycle.

Original language	English (US)
Pages (from-to)	13066-13071
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	23
DOIs	https://doi.org/10.1073/pnas.231487598
State	Published - Nov 6 2001

Keywords

Anaphase-promoting complex
Degradation
Ubiquitin

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.231487598

Cite this

@article{9b32733bb7ca4ed6be29e818748cd6be,

title = "Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities",

abstract = "CDC20/CDH1 activates the anaphase-promoting complex (APC) and targets various substrates for degradation, thereby allowing the ordered progression through mitosis and G1. We have found multiple functional CDH1 homologues in the chick. The transcripts of these novel genes are differentially localized to proliferating, differentiated, and postmitotic tissues. All four proteins bind and form a complex with APC in vitro and in cultural cells and have quantitatively different activities in mediating ubiquitination of various APC substrates. Our results suggest that multiple CDH1s may temporally and spatially regulate APC activity both within and outside of the cell cycle.",

keywords = "Anaphase-promoting complex, Degradation, Ubiquitin",

author = "Y. Wan and Kirschner, {M. W.}",

year = "2001",

month = nov,

day = "6",

doi = "10.1073/pnas.231487598",

language = "English (US)",

volume = "98",

pages = "13066--13071",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "23",

}

TY - JOUR

T1 - Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities

AU - Wan, Y.

AU - Kirschner, M. W.

PY - 2001/11/6

Y1 - 2001/11/6

N2 - CDC20/CDH1 activates the anaphase-promoting complex (APC) and targets various substrates for degradation, thereby allowing the ordered progression through mitosis and G1. We have found multiple functional CDH1 homologues in the chick. The transcripts of these novel genes are differentially localized to proliferating, differentiated, and postmitotic tissues. All four proteins bind and form a complex with APC in vitro and in cultural cells and have quantitatively different activities in mediating ubiquitination of various APC substrates. Our results suggest that multiple CDH1s may temporally and spatially regulate APC activity both within and outside of the cell cycle.

AB - CDC20/CDH1 activates the anaphase-promoting complex (APC) and targets various substrates for degradation, thereby allowing the ordered progression through mitosis and G1. We have found multiple functional CDH1 homologues in the chick. The transcripts of these novel genes are differentially localized to proliferating, differentiated, and postmitotic tissues. All four proteins bind and form a complex with APC in vitro and in cultural cells and have quantitatively different activities in mediating ubiquitination of various APC substrates. Our results suggest that multiple CDH1s may temporally and spatially regulate APC activity both within and outside of the cell cycle.

KW - Anaphase-promoting complex

KW - Degradation

KW - Ubiquitin

UR - http://www.scopus.com/inward/record.url?scp=0035818597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035818597&partnerID=8YFLogxK

U2 - 10.1073/pnas.231487598

DO - 10.1073/pnas.231487598

M3 - Article

C2 - 11687641

AN - SCOPUS:0035818597

SN - 0027-8424

VL - 98

SP - 13066

EP - 13071

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 23

ER -

Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this