Identification of novel spinal cholinergic genetic subtypes disclose chodl and Pitx2 as markers for fast motor neurons and partition cells

Anders Enjin, Nadine Rabe, Stan T. Nakanishi, Anna Vallstedt, Henrik Gezelius, Fatima Memic, Magnus Lind, Tord Hjalt, Warren G. Tourtellotte, Carl Bruder, Gregor Eichele, Patrick J. Whelan, Klas Kullander*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Spinal cholinergic neurons are critical for motor function in both the autonomic and somatic nervous systems and are affected in spinal cord injury and in diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy. Using two screening approaches and in situ hybridization, we identified 159 genes expressed in typical cholinergic patterns in the spinal cord. These include two general cholinergic neuron markers, one gene exclusively expressed in motor neurons, and nine genes expressed in unknown subtypes of somatic motor neurons. Further, we present evidence that chondrolectin (Chodl) is expressed by fast motor neurons and that estrogen-related receptor β (ERRβ) is a candidate marker for slow motor neurons. In addition, we suggest paired-like homeodomain transcription factor 2 (Pitx2) as a marker for cholinergic partition cells.

Original languageEnglish (US)
Pages (from-to)2284-2304
Number of pages21
JournalJournal of Comparative Neurology
Volume518
Issue number12
DOIs
StatePublished - Jun 15 2010

Keywords

  • Genetic screen
  • Interneuron
  • Motor neuron
  • Mouse genetics
  • Neuronal network
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

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