Abstract
In mammalian species, oocyte activation is initiated by oscillations in the intracellular concentration of free calcium ([Ca2+]i), which are also essential to allow embryonic development. To date, evidence supporting the hypothesis that a sperm factor is responsible for initiating oocyte activation has been presented in various mammalian species. Among the possible candidates to be the active sperm factor is the novel sperm-specific phospholipase C ζ (PLCζ), which besides its testis-specific expression is capable of initiating [Ca2+]i oscillations. In this study, we investigated the presence of PLCζ in the sperm of the domestic cat and whether normospermic and teratospermic cats differ in their PLCζ expression. Immunoblotting with anti-PLCζ antibodies confirmed the presence of an immunoreactive band of ~70 kDa in whole sperm lysates of domestic cat as well as in both soluble and "insoluble" fractions from this sperm. Additional immunoreactive bands, probably C- and N-terminal truncated versions of PLCζ, were also visualized in the soluble sperm fractions. Interestingly, immunoreactivity of PLCζ was detectable in teratospermic sperm, although with slightly less intensity than in normospermic sperm. In conclusion, domestic cat sperm express PLCζ in both cytosolic and high-pH fractions, which is consistent with data in other mammals. Sperm from teratospermic cats also express PLCζ, albeit at reduced concentrations, which may affect the fertility of these males.
Original language | English (US) |
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Pages (from-to) | 722-729 |
Number of pages | 8 |
Journal | Theriogenology |
Volume | 80 |
Issue number | 7 |
DOIs | |
State | Published - Oct 15 2013 |
Funding
This study was supported by a grant from the São Paulo Research Foundation (FAPESP) (06/59910-2; 07/53299-2) and the work in the laboratory of Dr. Rafael A. Fissore by a grant from the NIH ( HD051872 ). The authors thank Dr. Miriam H. Tsunemi, Dr. Andreza S. Figueiredo, and Dr. João Pessoa Araújo Junior for their help in this study.
Keywords
- Fertilization
- Immunoblotting
- Oocyte activation
- PLCζ
- Two-dimensional PAGE
ASJC Scopus subject areas
- Food Animals
- Small Animals
- Animal Science and Zoology
- Equine