Identification of polypeptide components of the Epstein-Barr virus early antigen complex with monoclonal antibodies

G. R. Pearson, B. Vroman, B. Chase, T. Sculley, M. Hummel, E. Kieff

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

Three monoclonal antibodies were produced against the Epstein-Barr virus-induced early antigen complex. These antibodies were shown to be specific for the early antigen complex by the fact that they reacted only with cells supporting a permissive or abortive Epstein-Barr virus infection and their synthesis was not affected by inhibitors of viral DNA synthesis. One monoclonal antibody, designated R3, was directed against a diffuse component of the early antigen complex since it reacted by immunofluorescence with cells fixed in acetone or methanol. The other two monoclonal antibodies, designated K8 and K9, reacted with a methanol-sensitive restricted component of this complex. The appearance of the R3 antigen in P3HR-1 superinfected Raji cells occurred approximately 4 h earlier than the antigen detected by K8. By both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and radioimmunoelectrophoresis, it was determined that the R3 monoclonal antibody recognized two major polypeptides with molecular weights of approximately 50,000 to 52,000, whereas K8 and K9 precipitated a protein of approximately 85,000. The R3 monoclonal antibody also immunoprecipitated an in vitro primary translation product. it was, therefore, possible to map this product to the Epstein-Barr virus DNA BamHI M fragment. These in vitro products were slightly smaller than the in vivo proteins, suggesting that these proteins probably undergo posttranslational modification during the virus replication cycle.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalUnknown Journal
Volume47
Issue number1
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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