Identification of sequences necessary for the association of cardiac myosin subunits

Elizabeth M. McNally*, Maria M. Bravo-Zehnder, Leslie A. Leinwand

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay.

Original languageEnglish (US)
Pages (from-to)585-590
Number of pages6
JournalJournal of Cell Biology
Issue number3
StatePublished - May 1991

ASJC Scopus subject areas

  • Cell Biology


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