TY - JOUR
T1 - Identification of sequences necessary for the association of cardiac myosin subunits
AU - McNally, Elizabeth M.
AU - Bravo-Zehnder, Maria M.
AU - Leinwand, Leslie A.
PY - 1991/5
Y1 - 1991/5
N2 - To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay.
AB - To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay.
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U2 - 10.1083/jcb.113.3.585
DO - 10.1083/jcb.113.3.585
M3 - Article
C2 - 2016338
AN - SCOPUS:0025894162
SN - 0021-9525
VL - 113
SP - 585
EP - 590
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -