Identification of the hepatic microbody membrane in freeze fracture replicas

J. K. Reddy, J. P. Tewari, D. J. Svoboda, S. K. Malhotra

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


A freeze fracture study of the microbody membrane was made in normal Csa male mouse liver and in livers of animals kept on diet supplemented with the hypolipidemic agents methyl 2 4 (p chlorophenyl)phenoxy 2 methyl propionate (methyl clofenapate) or ethyl α p chlorophenoxyisobutyrate. Livers of drug treated mice showed a marked increase in the number of microbodies in thin sections and this facilitated their identification in the freeze fracture replicas. Concave fracture (face a) of the microbody membrane both in normal and in drug treated livers showed numerous particles, often appearing to be grouped or arranged in an indistinct network. Convex fracture (face b) of the microbody membrane in normal liver was almost devoid of particles or showed few particles in aggregates. However, the corresponding fractures (face b) of the microbody membrane from livers of mice treated with either methyl clofenapate or chlorophenoxyisobutyrate showed numerous depressions, which appeared complementary to fracture face a (concave fracture). A resemblance between corresponding fractures of the microbody membrane and endoplasmic reticulum membrane is evident. Evidence of direct association between microbody membrane and endoplasmic reticulum is seen in the freeze fracture replicas, as previously reported through studies on thin sections.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalLaboratory Investigation
Issue number3
StatePublished - 1974

ASJC Scopus subject areas

  • General Medicine


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