Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow

Irene V. Budunova; Leonid A. Mittelman; Gennady A. Belitsky

doi:10.1007/BF00141066

Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow

Irene V. Budunova^*, Leonid A. Mittelman, Gennady A. Belitsky

^*Corresponding author for this work

Dermatology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca²⁺-ionophore A23187, butylated hydroxytoluene (BHT), dichlordiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.

Original language	English (US)
Pages (from-to)	77-89
Number of pages	13
Journal	Cell Biology and Toxicology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/BF00141066
State	Published - Jan 1989

Keywords

dye transfer
intercellular communication
tumor promoters

ASJC Scopus subject areas

Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

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10.1007/BF00141066

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title = "Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow",

abstract = "The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlordiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.",

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author = "Budunova, {Irene V.} and Mittelman, {Leonid A.} and Belitsky, {Gennady A.}",

year = "1989",

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T1 - Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow

AU - Budunova, Irene V.

AU - Mittelman, Leonid A.

AU - Belitsky, Gennady A.

PY - 1989/1

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N2 - The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlordiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.

AB - The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlordiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.

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Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow

Abstract

Keywords

ASJC Scopus subject areas

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