Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth

Andrea A. Duina, James A. Marsh, Richard F. Gaber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

We report the analysis of two Saccharomyces cerevisiae cyclophilins, Cpr6 and Cpr7, identified by their ability to interact in vivo with the transcriptional regulator Rpd3. Both cyclophilins have an extended carboxy-terminal region containing a three-unit tetratricopeptide repeat (TPR) motif and share significant amino acid identity with the mammalian cyclophilin CyP-40. Neither CPR6 nor CPR7 is essential but deletion of CPR7 results in a significant impairment of the rate of cell division. This is the first demonstration that a member of the cyclophilin family is required for normal cell growth. The nucleotide sequences encoding CPR6 and CPR7 have been deposited in GenBank (U48867 and U48868).

Original languageEnglish (US)
Pages (from-to)943-952
Number of pages10
JournalYeast
Volume12
Issue number10
DOIs
StatePublished - Aug 1996

Keywords

  • Budding yeast
  • Cyclophilin
  • Saccharomyces cerevisiae
  • Transcriptional regulator

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

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