Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth

Andrea A. Duina; James A. Marsh; Richard F. Gaber

doi:10.1002/(SICI)1097-0061(199608)12:10<943::AID-YEA997>3.0.CO;2-3

Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth

Andrea A. Duina, James A. Marsh, Richard F. Gaber^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

We report the analysis of two Saccharomyces cerevisiae cyclophilins, Cpr6 and Cpr7, identified by their ability to interact in vivo with the transcriptional regulator Rpd3. Both cyclophilins have an extended carboxy-terminal region containing a three-unit tetratricopeptide repeat (TPR) motif and share significant amino acid identity with the mammalian cyclophilin CyP-40. Neither CPR6 nor CPR7 is essential but deletion of CPR7 results in a significant impairment of the rate of cell division. This is the first demonstration that a member of the cyclophilin family is required for normal cell growth. The nucleotide sequences encoding CPR6 and CPR7 have been deposited in GenBank (U48867 and U48868).

Original language	English (US)
Pages (from-to)	943-952
Number of pages	10
Journal	Yeast
Volume	12
Issue number	10
DOIs	https://doi.org/10.1002/(SICI)1097-0061(199608)12:10<943::AID-YEA997>3.0.CO;2-3
State	Published - Aug 1996

Keywords

Budding yeast
Cyclophilin
Saccharomyces cerevisiae
Transcriptional regulator

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Genetics

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10.1002/(SICI)1097-0061(199608)12:10<943::AID-YEA997>3.0.CO;2-3

Cite this

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title = "Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth",

abstract = "We report the analysis of two Saccharomyces cerevisiae cyclophilins, Cpr6 and Cpr7, identified by their ability to interact in vivo with the transcriptional regulator Rpd3. Both cyclophilins have an extended carboxy-terminal region containing a three-unit tetratricopeptide repeat (TPR) motif and share significant amino acid identity with the mammalian cyclophilin CyP-40. Neither CPR6 nor CPR7 is essential but deletion of CPR7 results in a significant impairment of the rate of cell division. This is the first demonstration that a member of the cyclophilin family is required for normal cell growth. The nucleotide sequences encoding CPR6 and CPR7 have been deposited in GenBank (U48867 and U48868).",

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AU - Duina, Andrea A.

AU - Marsh, James A.

AU - Gaber, Richard F.

PY - 1996/8

Y1 - 1996/8

N2 - We report the analysis of two Saccharomyces cerevisiae cyclophilins, Cpr6 and Cpr7, identified by their ability to interact in vivo with the transcriptional regulator Rpd3. Both cyclophilins have an extended carboxy-terminal region containing a three-unit tetratricopeptide repeat (TPR) motif and share significant amino acid identity with the mammalian cyclophilin CyP-40. Neither CPR6 nor CPR7 is essential but deletion of CPR7 results in a significant impairment of the rate of cell division. This is the first demonstration that a member of the cyclophilin family is required for normal cell growth. The nucleotide sequences encoding CPR6 and CPR7 have been deposited in GenBank (U48867 and U48868).

AB - We report the analysis of two Saccharomyces cerevisiae cyclophilins, Cpr6 and Cpr7, identified by their ability to interact in vivo with the transcriptional regulator Rpd3. Both cyclophilins have an extended carboxy-terminal region containing a three-unit tetratricopeptide repeat (TPR) motif and share significant amino acid identity with the mammalian cyclophilin CyP-40. Neither CPR6 nor CPR7 is essential but deletion of CPR7 results in a significant impairment of the rate of cell division. This is the first demonstration that a member of the cyclophilin family is required for normal cell growth. The nucleotide sequences encoding CPR6 and CPR7 have been deposited in GenBank (U48867 and U48868).

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KW - Saccharomyces cerevisiae

KW - Transcriptional regulator

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Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth

Abstract

Keywords

ASJC Scopus subject areas

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