Identification, quantification, and localization of mRNA for three distinct alpha1 adrenergic receptor subtypes in human prostate

D. T. Price, D. A. Schwinn, J. W. Lomasney, L. F. Allen, M. G. Caron, R. J. Lefkowitz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

302 Scopus citations

Abstract

The dynamic component of bladder outlet obstruction caused by benign prostatic hyperplasia (BPH) is regulated by alpha1 adrenergic receptors (α1-AR) located in the prostatic stroma. Recently two α1-AR subtypes (α(1A), α(1B)) have been identified in the human prostate by both functional and pharmacological assays. However, the presence of the α(1C) subtype has not been evaluated, presumably due to the lack of availability of selective ligands for this receptor subtype. We have used molecular techniques to investigate the mRNA expression of all three α1-AR subtypes in the human prostate. RNA extracted from the prostate gland of 15 patients was used in ribonuclease protection assays to identify the expression of three α1-AR subtype mRNAs. Quantitative solution hybridization assays further identify the predominant subtype of α1-AR mRNA to be the α(1C), which represents approximately 70% of the total α1-AR mRNA in the human prostate. Furthermore, in situ hybridization localizes the α(1C) AR mRNA predominantly to the stromal compartment. The identification of a predominant α1-AR mRNA in human prostate identifies a potential need for subtype selective pharmaceutical agents. These agents could be very important clinically in the treatment of diseases such as BPH.

Original languageEnglish (US)
Pages (from-to)546-551
Number of pages6
JournalJournal of Urology
Volume150
Issue number2
DOIs
StatePublished - 1993

Keywords

  • prostate
  • prostatic diseases
  • prostatic hypertrophy
  • receptors, adrenergic

ASJC Scopus subject areas

  • Urology

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