Abstract
Viral DNA genomes replicating in cells encounter a myriad of host factors that facilitate or hinder viral replication. Viral proteins expressed early during infection modulate host factors interacting with viral genomes, recruiting proteins to promote viral replication, and limiting access to antiviral repressors. Although some host factors manipulated by viruses have been identified, we have limited knowledge of pathways exploited during infection and how these differ between viruses. To identify cellular processes manipulated during viral replication, we defined proteomes associated with viral genomes during infection with adenovirus, herpes simplex virus and vaccinia virus. We compared enrichment of host factors between virus proteomes and confirmed association with viral genomes and replication compartments. Using adenovirus as an illustrative example, we uncovered host factors deactivated by early viral proteins, and identified a subgroup of nucleolar proteins that aid virus replication. Our data sets provide valuable resources of virus-host interactions that affect proteins on viral genomes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2079-2097 |
| Number of pages | 19 |
| Journal | Molecular and Cellular Proteomics |
| Volume | 16 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2017 |
Funding
* This work was supported by grants to M.D.W. from the National Institutes of Health (NS082240, CA097093 and AI115104), a Catalyst Grant from the Institute for Immunology of the University of Pennsylvania, and the Foerderer Award from the Children’s Hospital of Philadelphia. B.A.G acknowledges funding from the National Institutes of Health (AI118891 and GM110174). E.D.R. was supported by an Academic Diversity Postdoctoral Fellowship from the Office of the Vice Provost for Research at the University of Pennsylvania and by an NIH Research Supplement to Support Diversity (NS082240). N.J.P. was supported in part by T32 NS007180. D.C.A. was supported in part by T32 CA115299 and F32 GM112414. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. □S This article contains supplemental material. ¶¶ To whom correspondence should be addressed: Children’s Hospital of Philadelphia, 4050 Colket Translational Research Building 3501 Civic Center Blvd, Philadelphia, PA 19104. Tel.: 1-267-4252068; E-mail: [email protected].
ASJC Scopus subject areas
- Analytical Chemistry
- Molecular Biology
- Biochemistry
