Identifying tumor cell growth inhibitors by combinatorial chemistry and zebrafish assays

Jing Xiang*, Hongbo Yang, Chao Che, Haixia Zou, Hanshuo Yang, Yuquan Wei, Junmin Quan, Hui Zhang, Zhen Yang, Shuo Lin

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Cyclin-dependent kinases (CDKs) play important roles in regulating cell cycle progression, and altered cell cycles resulting from over-expression or abnormal activation of CDKs observed in many human cancers. As a result, CDKs have become extensive studied targets for developing chemical inhibitors for cancer therapies; however, protein kinases share a highly conserved ATP binding pocket at which most chemical inhibitors bind, therefore, a major challenge in developing kinase inhibitors is achieving target selectivity. To identify cell growth inhibitors with potential applications in cancer therapy, we used an integrated approach that combines one-pot chemical synthesis in a combinatorial manner to generate diversified small molecules with new chemical scaffolds coupled with growth inhibition assay using developing zebrafish embryos. We report the successful identification of a novel lead compound that displays selective inhibitory effects on CDK2 activity, cancer cell proliferation, and tumor progression in vivo. Our approaches should have general applications in developing cell proliferation inhibitors using an efficient combinatorial chemical genetic method and integrated biological assays. The novel cell growth inhibitor we identified should have potential as a cancer therapeutic agent.

Original languageEnglish (US)
Article numbere4361
JournalPloS one
Volume4
Issue number2
DOIs
StatePublished - Feb 5 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Xiang, J., Yang, H., Che, C., Zou, H., Yang, H., Wei, Y., Quan, J., Zhang, H., Yang, Z., & Lin, S. (2009). Identifying tumor cell growth inhibitors by combinatorial chemistry and zebrafish assays. PloS one, 4(2), [e4361]. https://doi.org/10.1371/journal.pone.0004361