IgY antiporcine endothelial cell antibodies effectively block human antiporcine xenoantibody binding

Jonathan Fryer*, Joseph Firca, Joseph Leventhal, Beth Blondin, Andrew Malcolm, David Ivancic, Ripal Gandhi, Ateet Shah, Winnie Pao, Michael Abecassis, Dixon Kaufman, Frank Stuart, Byron Anderson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Avian IgY antibodies are structurally different from mammalian IgGs and do not fix mammalian complement components or bind human Fc receptors. As these antibody-mediated interactions are believed to play significant roles in both hyperacute rejection (HAR) and acute vascular xenograft rejection (AVXR), IgY antibodies to xenoantigen target epitopes may inhibit these rejection processes. In this report, we show that chicken IgY antibodies to α-Gal antigen epitopes and to other porcine aortic endothelial cell (PAEC) antigens block human xenoreactive natural antibody binding to both porcine and rat cardiac tissues and porcine kidney tissues. Chicken IgY antibodies blocked complement-mediated lysis of PAECs by human serum, and inhibited antibody-dependent cell-mediated lysis of PAECs by heat-inactivated human serum plus peripheral blood leukocytes. Binding of IgY to porcine endothelial cells did not affect cell morphology nor expression of E-selectin. These results suggest that avian IgYs could be of potential use in inhibiting pig- to-human xenograft rejection.

Original languageEnglish (US)
Pages (from-to)98-109
Number of pages12
JournalXenotransplantation
Volume6
Issue number2
DOIs
StatePublished - May 1999

Keywords

  • Endothelial activation
  • Endothelial cells
  • IgY
  • Xenoantibodies
  • Xenotransplantation

ASJC Scopus subject areas

  • Immunology
  • Transplantation

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