IL-24 modulates IFN-γ expression in patients with tuberculosis

Bo Wu, Chunhong Huang, Midori Kato-Maeda, Philip C. Hopewell, Charles L. Daley, Alan M. Krensky, Carol Clayberger*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

IL-24 is a newly described member of the IL-10 family. We previously demonstrated that PBMC from TB patients exhibited low levels of IL-24 and IFN-γ compared to subjects with latent tuberculosis infection (LTBI). In order to investigate the role of IL-24 in IFN-γ expression in TB patients, we stimulated PBMC from individuals with LTBI or TB patients with the Mtb-specific antigen, early secretory antigenic target-6 (ESAT-6) and measured cytokine expression using quantitative real-time PCR (qPCR). Exogenous IL-24 increased IFN-γ expression in PBMC obtained from TB patients while neutralization of IL-24 reduced IFN-γ expression in PBMC from subjects with LTBI. Exogenous IL-24 enhanced IFN-γ expression by increasing expression of IL-12 family cytokines, including IL-12α, IL-12β, IL-23α and IL-27, and by reducing FOXP3 expression in PBMC from TB patients. This is the first demonstration that IL-24 may play an important role in IFN-γ expression following infection with Mtb.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalImmunology Letters
Volume117
Issue number1
DOIs
StatePublished - Apr 15 2008

Keywords

  • Cytokine
  • Human
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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    Wu, B., Huang, C., Kato-Maeda, M., Hopewell, P. C., Daley, C. L., Krensky, A. M., & Clayberger, C. (2008). IL-24 modulates IFN-γ expression in patients with tuberculosis. Immunology Letters, 117(1), 57-62. https://doi.org/10.1016/j.imlet.2007.11.018