Imaging hemodynamic response after ischemic stroke in mouse cortex using visible-light optical coherence tomography

Siyu Chen, Qi Liu, Xiao Shu, Brian Soetikno, Shanbao Tong, Hao F. Zhang

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Visible-light optical coherence tomography (Vis-OCT) is an emerging technology that can image hemodynamic response in microvasculature. Vis-OCT can retrieve blood oxygen saturation (sO2) mapping using intrinsic optical absorption contrast while providing high-resolution anatomical vascular structures at the same time. To improve the accuracy of Vis-OCT oximetry on vessels embedded in highly scattering medium, i.e., brain cortex, we developed and formulated a novel dual-depth sampling and normalization strategy that allowed us to minimize the detrimental effect of ubiquitous tissue scattering. We applied our newly developed approach to monitor the hemodynamic response in mouse cortex after focal photothrombosis. We observed vessel dilatation, which was negatively correlated with the original vessel diameter, in the penumbra region. The sO2 of vessels in the penumbra region also dropped below normal range after focal ischemia.

Original languageEnglish (US)
Article number#266912
Pages (from-to)3377-3390
Number of pages14
JournalBiomedical Optics Express
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2016

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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