Imaging human epithelial properties with polarized light- scattering spectroscopy

Rajan S. Gurjar; Vadim Backman; Lev T. Perelman; Irene Georgakoudi; Kamran Badizadegan; Irving Itzkan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1038/nm1101-1245

Imaging human epithelial properties with polarized light- scattering spectroscopy

Rajan S. Gurjar, Vadim Backman^*, Lev T. Perelman, Irene Georgakoudi, Kamran Badizadegan, Irving Itzkan, Ramachandra R. Dasari, Michael S. Feld

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

396 Scopus citations

Abstract

Biomedical imaging with light-scattering spectroscopy (LSS) is a novel optical technology developed to probe the structure of living epithelial cells in situ without need for tissue removal. LSS makes it possible to distinguish between single backscattering from epithelial-cell nuclei and multiply scattered light. The spectrum of the single backscattering component is further analyzed to provide quantitative information about the epithelial-cell nuclei such as nuclear size, degree of pleomorphism, degree of hyperchromasia and amount of chromatin. LSS imaging allows mapping these histological properties over wide areas of epithelial lining. Because nuclear enlargement, pleomorphism and hyperchromasia are principal features of nuclear atypia associated with precancerous and cancerous changes in virtually all epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.

Original language	English (US)
Pages (from-to)	1245-1248
Number of pages	4
Journal	Nature Medicine
Volume	7
Issue number	11
DOIs	https://doi.org/10.1038/nm1101-1245
State	Published - 2001

Funding

Acknowledgments We thank W. Lencer for T84 cells and S. Fulghum for useful comments. This study was supported by NIH grant P41-RR02594, NIH grant R01-CA53717 and CIMIT grant 731-3489-5.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/nm1101-1245

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@article{66da2a1fa8e94659a4eda9bb5da52251,

title = "Imaging human epithelial properties with polarized light- scattering spectroscopy",

abstract = "Biomedical imaging with light-scattering spectroscopy (LSS) is a novel optical technology developed to probe the structure of living epithelial cells in situ without need for tissue removal. LSS makes it possible to distinguish between single backscattering from epithelial-cell nuclei and multiply scattered light. The spectrum of the single backscattering component is further analyzed to provide quantitative information about the epithelial-cell nuclei such as nuclear size, degree of pleomorphism, degree of hyperchromasia and amount of chromatin. LSS imaging allows mapping these histological properties over wide areas of epithelial lining. Because nuclear enlargement, pleomorphism and hyperchromasia are principal features of nuclear atypia associated with precancerous and cancerous changes in virtually all epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.",

author = "Gurjar, {Rajan S.} and Vadim Backman and Perelman, {Lev T.} and Irene Georgakoudi and Kamran Badizadegan and Irving Itzkan and Dasari, {Ramachandra R.} and Feld, {Michael S.}",

note = "Funding Information: Acknowledgments We thank W. Lencer for T84 cells and S. Fulghum for useful comments. This study was supported by NIH grant P41-RR02594, NIH grant R01-CA53717 and CIMIT grant 731-3489-5.",

year = "2001",

doi = "10.1038/nm1101-1245",

language = "English (US)",

volume = "7",

pages = "1245--1248",

journal = "Nature Medicine",

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publisher = "Nature Publishing Group",

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T1 - Imaging human epithelial properties with polarized light- scattering spectroscopy

AU - Gurjar, Rajan S.

AU - Backman, Vadim

AU - Perelman, Lev T.

AU - Georgakoudi, Irene

AU - Badizadegan, Kamran

AU - Itzkan, Irving

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

N1 - Funding Information: Acknowledgments We thank W. Lencer for T84 cells and S. Fulghum for useful comments. This study was supported by NIH grant P41-RR02594, NIH grant R01-CA53717 and CIMIT grant 731-3489-5.

PY - 2001

Y1 - 2001

N2 - Biomedical imaging with light-scattering spectroscopy (LSS) is a novel optical technology developed to probe the structure of living epithelial cells in situ without need for tissue removal. LSS makes it possible to distinguish between single backscattering from epithelial-cell nuclei and multiply scattered light. The spectrum of the single backscattering component is further analyzed to provide quantitative information about the epithelial-cell nuclei such as nuclear size, degree of pleomorphism, degree of hyperchromasia and amount of chromatin. LSS imaging allows mapping these histological properties over wide areas of epithelial lining. Because nuclear enlargement, pleomorphism and hyperchromasia are principal features of nuclear atypia associated with precancerous and cancerous changes in virtually all epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.

AB - Biomedical imaging with light-scattering spectroscopy (LSS) is a novel optical technology developed to probe the structure of living epithelial cells in situ without need for tissue removal. LSS makes it possible to distinguish between single backscattering from epithelial-cell nuclei and multiply scattered light. The spectrum of the single backscattering component is further analyzed to provide quantitative information about the epithelial-cell nuclei such as nuclear size, degree of pleomorphism, degree of hyperchromasia and amount of chromatin. LSS imaging allows mapping these histological properties over wide areas of epithelial lining. Because nuclear enlargement, pleomorphism and hyperchromasia are principal features of nuclear atypia associated with precancerous and cancerous changes in virtually all epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.

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JO - Nature Medicine

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ER -

Imaging human epithelial properties with polarized light- scattering spectroscopy

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

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