Imaging human epithelial properties with polarized light- scattering spectroscopy

Rajan S. Gurjar, Vadim Backman*, Lev T. Perelman, Irene Georgakoudi, Kamran Badizadegan, Irving Itzkan, Ramachandra R. Dasari, Michael S. Feld

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

392 Scopus citations


Biomedical imaging with light-scattering spectroscopy (LSS) is a novel optical technology developed to probe the structure of living epithelial cells in situ without need for tissue removal. LSS makes it possible to distinguish between single backscattering from epithelial-cell nuclei and multiply scattered light. The spectrum of the single backscattering component is further analyzed to provide quantitative information about the epithelial-cell nuclei such as nuclear size, degree of pleomorphism, degree of hyperchromasia and amount of chromatin. LSS imaging allows mapping these histological properties over wide areas of epithelial lining. Because nuclear enlargement, pleomorphism and hyperchromasia are principal features of nuclear atypia associated with precancerous and cancerous changes in virtually all epithelia, LSS imaging can be used to detect precancerous lesions in optically accessible organs.

Original languageEnglish (US)
Pages (from-to)1245-1248
Number of pages4
JournalNature Medicine
Issue number11
StatePublished - 2001

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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