Imaging of hemoglobin oxygen saturation variations in single vessels in vivo using photoacoustic microscopy

Hao F. Zhang, Konstantin Maslov, Mathangi Sivaramakrishnan, Gheorghe Stoica, Lihong V. Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

332 Scopus citations

Abstract

Photoacoustic microscopy was used to noninvasively image variations in hemoglobin oxygen saturation (S O2) in the subcutaneous microvasculature of rats in vivo. In phantom tests, the calculated concentration fractions of red ink in double-ink mixtures matched the actual values with a 1% error. In ex vivo studies, the calculated S O2 in bovine blood agreed with the standard spectrophotometric measurements within a 4% systematic difference. In in vivo studies, arteries and veins were separated based on the measured S O2 values and variations in S O2 between different physiological states (hyperoxia, normoxia, and hypoxia) were imaged in single blood vessels.

Original languageEnglish (US)
Article number053901
JournalApplied Physics Letters
Volume90
Issue number5
DOIs
StatePublished - 2007

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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