Immortalization of male genital duct epithelium: An assay system for the cystic fibrosis gene

Lindsay Coleman*, Ann Harris

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The epithelia lining the vas deferens and epididymis are directly involved in the pathology of the autosomal recessive disease cystic fibrosis (CF). We have established culture systems for these epithelial cells. Long-term cell lines have now been generated from these primary epithelial cells by transformation with a plasmid containing an origin-defective simian virus 40 (SV40). Lines have been established from vas deferens and epididymis and both maintain expression of the CF gene.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalJournal of Cell Science
Volume98
Issue number1
StatePublished - Jan 1 1991

Keywords

  • Cystic fibrosis
  • Epididymis
  • Epithelium
  • Immortalization
  • Vas deferens

ASJC Scopus subject areas

  • Cell Biology

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