Immunobiological consequence of immunization of female mice with homologous spermatozoa: Induction of infertility

Kenneth S.K. Tung*, Ellen H. Goldberg, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Female Swiss Webster mice were immunized intraperitoneally with mouse epididymal spermatozoa or with phosphate buffered saline (PBS) and their fertility was compared by (1) incidence and size of litters, (2) number of uterine implantation sites, and (3) incidence and number of fertilized eggs in the oviducts. Statistically significant reduction in fertility was noted following two courses of injections of spermatozoa; 12% of mice injected with spermatozoa had litters compared with 80% of mice injected with PBS. The infertility did not seem to be related to a failure in fertilization since the two groups of mice had a similar incidence and number of fertilized eggs in the oviducts. All female mice were found to have a 'natural' anti-acrosomal antibody. Following immunization with spermatozoa, antibodies to 'postacrosomal' region, the main piece and the midpiece of the tail, as well as cytotoxic antisperm antibodies, appeared. Anti-LDH-X antibody was not detected. However, correlation was not found between infertility and antisperm antibodies or sperm granulomata that developed in the peritoneal cavities. It is concluded that female mice receiving repeated i.p. injections of mouse spermatozoa become infertile and that the infertility is related to interference with events after fertilization.

Original languageEnglish (US)
Pages (from-to)145-158
Number of pages14
JournalJournal of Reproductive Immunology
Volume1
Issue number3
DOIs
StatePublished - Oct 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Immunobiological consequence of immunization of female mice with homologous spermatozoa: Induction of infertility'. Together they form a unique fingerprint.

  • Cite this