Immunochemical studies on the enkephalins and endorphins

R. P. Schleimer, C. Y. Leung, W. J. Peterson, E. Benjamini

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Leucine-enkephalin antibodies have been used for the elucidation of the immunochemical relationships among enkephalins, endorphins and related compounds. Data presented in this report indicate that immunization of a rabbit with an immunogen consisting of BGG-(Ala)3-leu-enkephalin induced antibodies capable of specifically binding with leu-enkephalin, with an association constant (K0) of 1.9 x 105 L/M. Since the leu-enkephalin was conjugated to BGG through an (Ala)3 spacer, it is possible that leu-enkephalin represents only part of the antigenic determinant. Data presented show that indeed (Ala)3 leu-enkephalin binds with antibodies 150 times better, than does leu-enkephalin. This conclusion is based on the finding that (Ala)3 leu-enkephalin was 150 times more efficient in inhibiting the reaction between the antibodies (anti BGG-(Ala)3 leu-enkephalin) and [3H]-leu-enkephalin.

Original languageEnglish (US)
Pages (from-to)235-240
Number of pages6
JournalProceedings of the Western Pharmacology Society
VolumeVOL. 22
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Pharmacology


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