TY - JOUR
T1 - Immunochip analysis identifies multiple susceptibility loci for systemic sclerosis
AU - Mayes, Maureen D.
AU - Bossini-Castillo, Lara
AU - Gorlova, Olga
AU - Martin, José Ezequiel
AU - Zhou, Xiaodong
AU - Chen, Wei V.
AU - Assassi, Shervin
AU - Ying, Jun
AU - Tan, Filemon K.
AU - Arnett, Frank C.
AU - Reveille, John D.
AU - Guerra, Sandra
AU - Teruel, María
AU - Carmona, Francisco David
AU - Gregersen, Peter K.
AU - Lee, Annette T.
AU - López-Isac, Elena
AU - Ochoa, Eguzkine
AU - Carreira, Patricia
AU - Simeón, Carmen Pilar
AU - Castellví, Iván
AU - González-Gay, Miguel Ángel
AU - Zhernakova, Alexandra
AU - Padyukov, Leonid
AU - Alarcón-Riquelme, Marta
AU - Wijmenga, Cisca
AU - Brown, Matthew
AU - Beretta, Lorenzo
AU - Riemekasten, Gabriela
AU - Witte, Torsten
AU - Hunzelmann, Nicolas
AU - Kreuter, Alexander
AU - Distler, Jörg H.W.
AU - Voskuyl, Alexandre E.
AU - Schuerwegh, Annemie J.
AU - Hesselstrand, Roger
AU - Nordin, Annika
AU - Airó, Paolo
AU - Lunardi, Claudio
AU - Shiels, Paul
AU - Van Laar, Jacob M.
AU - Herrick, Ariane
AU - Worthington, Jane
AU - Denton, Christopher
AU - Wigley, Fredrick M.
AU - Hummers, Laura K.
AU - Varga, John
AU - Hinchcliff, Monique E.
AU - Baron, Murray
AU - Hudson, Marie
N1 - Funding Information:
This study was supported by National Institutes of Health (NIH) National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) Centers of Research Translation (CORT) grant P50AR054144, NIH grant KL2RR024149-04, NIH NIAMS grant N01-AR02251, and NIH National Center for Research Resources grant 3UL1RR024148. This study was supported by GEN-FER from the Spanish Society of Rheumatology, SAF2009-11110 and SAF2012-34435 from the Spanish Ministry of Science, CTS-4977 from Junta de Andalucia, and RD08/0075 and RD12/0009/0004 from the Redes Temáticas de Investigación Cooperativa en Salud (Red de Investigación en Inflamación y Enfermedades Reumáticas). We are indebted to the Immunochip Consortium for provision of the control data. We are grateful to Julio Charles, Marilyn Perry, Suzanne S. Taillefer (National Study Coordinator for the Canadian Scleroderma Research Group), Sofia Vargas, Sonia García, and Gema Robledo. We also thank the Scleroderma Foundation, the Spanish Scleroderma Patient Group, the Canadian Scleroderma Patient Group, the Federation of European Scleroderma Associations, National DNA Bank Carlos III (University of Salamanca), the European League against Rheumatism Scleroderma Trials and Research group, the German Network of Systemic Sclerosis, and the affected individuals, friends, spouses, and others who generously provided the samples for these studies.
PY - 2014/1/2
Y1 - 2014/1/2
N2 - In this study, 1,833 systemic sclerosis (SSc) cases and 3,466 controls were genotyped with the Immunochip array. Classical alleles, amino acid residues, and SNPs across the human leukocyte antigen (HLA) region were imputed and tested. These analyses resulted in a model composed of six polymorphic amino acid positions and seven SNPs that explained the observed significant associations in the region. In addition, a replication step comprising 4,017 SSc cases and 5,935 controls was carried out for several selected non-HLA variants, reaching a total of 5,850 cases and 9,401 controls of European ancestry. Following this strategy, we identified and validated three SSc risk loci, including DNASE1L3 at 3p14, the SCHIP1-IL12A locus at 3q25, and ATG5 at 6q21, as well as a suggested association of the TREH-DDX6 locus at 11q23. The associations of several previously reported SSc risk loci were validated and further refined, and the observed peak of association in PXK was related to DNASE1L3. Our study has increased the number of known genetic associations with SSc, provided further insight into the pleiotropic effects of shared autoimmune risk factors, and highlighted the power of dense mapping for detecting previously overlooked susceptibility loci.
AB - In this study, 1,833 systemic sclerosis (SSc) cases and 3,466 controls were genotyped with the Immunochip array. Classical alleles, amino acid residues, and SNPs across the human leukocyte antigen (HLA) region were imputed and tested. These analyses resulted in a model composed of six polymorphic amino acid positions and seven SNPs that explained the observed significant associations in the region. In addition, a replication step comprising 4,017 SSc cases and 5,935 controls was carried out for several selected non-HLA variants, reaching a total of 5,850 cases and 9,401 controls of European ancestry. Following this strategy, we identified and validated three SSc risk loci, including DNASE1L3 at 3p14, the SCHIP1-IL12A locus at 3q25, and ATG5 at 6q21, as well as a suggested association of the TREH-DDX6 locus at 11q23. The associations of several previously reported SSc risk loci were validated and further refined, and the observed peak of association in PXK was related to DNASE1L3. Our study has increased the number of known genetic associations with SSc, provided further insight into the pleiotropic effects of shared autoimmune risk factors, and highlighted the power of dense mapping for detecting previously overlooked susceptibility loci.
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U2 - 10.1016/j.ajhg.2013.12.002
DO - 10.1016/j.ajhg.2013.12.002
M3 - Article
C2 - 24387989
AN - SCOPUS:84891817885
SN - 0002-9297
VL - 94
SP - 47
EP - 61
JO - American journal of human genetics
JF - American journal of human genetics
IS - 1
ER -