Immunocytochemical localization of enkephalin and substance P in the dorsal tegmental nuclei in human fetal brain

Virginia M. Pickel*, Khushdev K. Sumal, Donald J. Reis, Richard J. Miller, Anti Hervonen

*Corresponding author for this work

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The peroxidase‐antiperoxidase (PAP) technique is used to examine the localization of methionine [Met5]‐enkephalin and substance P in the dorsal tegmental nuclei of the human fetal brain. Specific antiserum to both peptides is immunocytochemically detectable in neuronal perikarya and varicose processes in the human dorsal tegmental nuclei at 17–21 weeks of gestation. However the two peptides show a differential distribution in the central versus peripheral portion of the nucleus. Enkephalin‐like immunoreactivity is primarily localized in perikarya along the peripheral borders of the tegmental nuclei, whereas substance P is present primarily in varicose terminals and a few perikarya in the more central portions. These findings suggest that substance P‐containing terminals in dorsal tegmental nuclei are probably derived from afferent axons and that enkephalin is present in intrinsic neurons.

Original languageEnglish (US)
Pages (from-to)805-814
Number of pages10
JournalJournal of Comparative Neurology
Volume193
Issue number3
DOIs
StatePublished - Jan 1 1980

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Enkephalins
Substance P
Methionine Enkephalin
Peptides
Brain
Axons
Immune Sera
Neurons
Pregnancy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pickel, Virginia M. ; Sumal, Khushdev K. ; Reis, Donald J. ; Miller, Richard J. ; Hervonen, Anti. / Immunocytochemical localization of enkephalin and substance P in the dorsal tegmental nuclei in human fetal brain. In: Journal of Comparative Neurology. 1980 ; Vol. 193, No. 3. pp. 805-814.
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abstract = "The peroxidase‐antiperoxidase (PAP) technique is used to examine the localization of methionine [Met5]‐enkephalin and substance P in the dorsal tegmental nuclei of the human fetal brain. Specific antiserum to both peptides is immunocytochemically detectable in neuronal perikarya and varicose processes in the human dorsal tegmental nuclei at 17–21 weeks of gestation. However the two peptides show a differential distribution in the central versus peripheral portion of the nucleus. Enkephalin‐like immunoreactivity is primarily localized in perikarya along the peripheral borders of the tegmental nuclei, whereas substance P is present primarily in varicose terminals and a few perikarya in the more central portions. These findings suggest that substance P‐containing terminals in dorsal tegmental nuclei are probably derived from afferent axons and that enkephalin is present in intrinsic neurons.",
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Immunocytochemical localization of enkephalin and substance P in the dorsal tegmental nuclei in human fetal brain. / Pickel, Virginia M.; Sumal, Khushdev K.; Reis, Donald J.; Miller, Richard J.; Hervonen, Anti.

In: Journal of Comparative Neurology, Vol. 193, No. 3, 01.01.1980, p. 805-814.

Research output: Contribution to journalArticle

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