Immunocytochemical localization of retinoid-binding protein in the dogfish retina

Vijay Sarthy*, Corey Bickoff

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Purpose. The cellular localization of retinoid-binding proteins has been extensively studied in the mammalian retina. Less is known about the distribution and identity of retinoid-binding proteins in lower vertebrate retinas. To understand their cellular functions, we have examined the distribution of specific retinoid-binding proteins in the neural retina and RPE of an elasmobranch, the dogfish. Methods. Retinoid-binding proteins were localized in cryostat sections of dogfish eyes by the indirect immunofluorescence technique. We used well-characterized antibodies that have been previously employed for immunostaining. Immunoblotting was carried out to identify the antigen. Results. The studies show that the cellular retinaldehyde-binding protein is present in the RPE and retinal Muller cells, and the interphotoreceptor retinoid-binding protein is found in the interstitial space between pigment epithelium and retina. Conclusions. The distribution of retinoid-binding proteins in dogfish retina is similar to that found in the mammalian retina which suggests that the sites of action of the retinoid-binding proteins are highly conserved in vertebrates.

Original languageEnglish (US)
Pages (from-to)905-907
Number of pages3
JournalCurrent eye research
Volume15
Issue number8
DOIs
StatePublished - Aug 1996

Keywords

  • Elasmobranch
  • Muller cell
  • Retinal pigment epithelium
  • Retinoid-binding proteins
  • Vitamin A

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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