Immunocytochemical localization of urate oxidase, fatty acyl-CoA oxidase, and catalase in bovine kidney peroxisomes

N. Usuda, M. I. Usman, M. K. Reddy, T. Hashimoto, Janardan K Reddy, Sambasiva Rao Musunuri

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We investigated the localization of urate oxidase, peroxisomal fatty acyl-CoA oxidase, and catalase in bovine kidney by immunoblot analysis and protein A-gold immunocytochemistry, using the respective polyclonal monospecific antibodies raised against the enzymes purified from rat liver. By immunoblot analysis, these three proteins were detected in bovine kidney and bovine liver homogenates. Subcellular localization of these three enzymes in kidney was ascertained by protein A-gold immunocytochemical staining of Lowicryl K4M-embedded tissue. Peroxisomes in bovine kidney cortical epithelium possessed crystalloid cores or nucleoids, which were found to be the exclusive sites of urate oxidase localization. The limiting membrane, the marginal plate, and the matrix or renal peroxisomes were negative for urate oxidase staining. In contrast, catalase and fatty acyl-CoA oxidase were found in the peroxisome matrix. These results demonstrate that, unlike rat kidney peroxisomes which lack urate oxidase, peroxisomes of bovine kidney contain this enzyme as well as peroxisomal fatty acyl-CoA oxidase.

Original languageEnglish (US)
Pages (from-to)253-258
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume36
Issue number3
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Anatomy
  • Histology

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