Immunogold labelling of the intermediate filamentlaminanuclear matrix system in hela and bhk21 cells

Wholemount, sequentially extracted cells combined with immunogold electron microscopy were developed to demonstrate the intermediate filaments, lamina, and nuclear matrix (IFLNM) and to identify their protein components. The IFs of HeLa cells were reacted both with keratin and vimentin monoclonal antibodies; meanwhile, the IF network of BHK21 cell was reacted only with vimentin monoclonal antibody. The lamina and nuclear matrices of both HeLa and BHK21 cell were labelled, respectively, with lamin monoclonal antibodygold complex and 280 Kd nuclear matrix protein monoclonal antibodygold complex. The monoclonal antibody to keratin could crossreact with the lamina both of HeLa and BHK21 cells. Copyright1991 WileyLiss, Inc.