Immunogold labelling of the intermediate filamentlaminanuclear matrix system in hela and bhk21 cells

Renjie Jiao*, Donglan Wu, Bo Zhang, Shutao Cai, Zhonghe Zhai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Wholemount, sequentially extracted cells combined with immunogold electron microscopy were developed to demonstrate the intermediate filaments, lamina, and nuclear matrix (IFLNM) and to identify their protein components. The IFs of HeLa cells were reacted both with keratin and vimentin monoclonal antibodies; meanwhile, the IF network of BHK21 cell was reacted only with vimentin monoclonal antibody. The lamina and nuclear matrices of both HeLa and BHK21 cell were labelled, respectively, with lamin monoclonal antibodygold complex and 280 Kd nuclear matrix protein monoclonal antibodygold complex. The monoclonal antibody to keratin could crossreact with the lamina both of HeLa and BHK21 cells. Copyright1991 WileyLiss, Inc.

Original languageEnglish (US)
Pages (from-to)126-134
Number of pages9
JournalJournal of Electron Microscopy Technique
Volume18
Issue number2
DOIs
StatePublished - Jun 1991

Keywords

  • Colloidal gold
  • Crossreaction
  • Monoclonal antibody
  • Sequential extraction
  • Wholemount cell

ASJC Scopus subject areas

  • Anatomy

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