Immunohistochemical detection with quantum dots

Rizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio, Kevin A. Roth

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations

Abstract

Quantum dot (QD) conjugates have many immunohistochemical applications. The optical, excitation/emission, and photostable properties of QDs offer several advantages over the use of chromogens or organic fluorophores in these applications. Here, we describe the use of QD conjugates to detect primary antibody binding in fixed tissue sections. We also describe the use of QDs in simultaneous and sequential multilabeling procedures and in combination with enzyme-based signal amplification techniques. QD conjugates expand the arsenal of the immunohistochemist and increase experimental flexibility in many applications.

Original languageEnglish (US)
Title of host publicationQuantum Dots
Subtitle of host publicationApplications in Biology
EditorsMarcel P. Bruchez, Charles Z. Hotz
Pages11-28
Number of pages18
DOIs
StatePublished - Feb 2 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume374
ISSN (Print)1064-3745

Keywords

  • Antibody binding
  • Detection methods
  • Fluorophore
  • Immunostaining
  • Multi-labeling
  • Tyramide signal amplification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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