Immunohistochemical localization of epidermal growth factor and transforming growth factor α in the male rat accessory sex organs

H. H.H. Wu, H. Kawamata, K. Kawai, C. Lee, R. Oyasu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We examined the presence of epidermal growth factor (EGF) and transforming growth factor α (TGFα) in the prostate (ventral, lateral, dorsal lobes), coagulating gland and seminal vesicle of Fisher 344 adult male rats by immunohistochemical and radioimmunoassay methods. Immunohistochemically demonstrable EGF was localized in the luminal secretion only of the dorsal lobe of the prostate. Reactive TGFα was localized in the lateral lobe (100% of epithelial cells), dorsal lobe (about 40% of the epithelial cells) and seminal vesicle (100% of epithelial cells), but not in the coagulating gland or ventral lobe of the prostate. Radioimmunoassay also demonstrated a measurable amount of TGFα in the lateral lobe (194 pg./gm. wet weight) and seminal vesicle (74 pg./gm.). Assayable EGF was demonstrated at much higher levels in all prostate lobes (ranging from 1.2 μg./gm. wet weight in the ventral lobe to 26.4 μg./gm. in the dorsal lobe) and the seminal vesicle (0.9 μg./gm.). This is the first report describing the presence of immunoreactive TGFα and EGF in the male accessory sex organs.

Original languageEnglish (US)
Pages (from-to)990-993
Number of pages4
JournalJournal of Urology
Volume150
Issue number3
DOIs
StatePublished - 1993

Keywords

  • epidermal growth factor- urogastrone
  • immunohistochemistry
  • prostate
  • radioimmunoassay
  • transforming growth factor alpha

ASJC Scopus subject areas

  • Urology

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