Immunohistochemical localization of LDH-X during spermatogenesis in mouse testes

Marie Hintz*, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Testis-specific lactate dehydrogenase, LDH-X, was localized spatially and temporally in the germinal epithelium of the mouse by the indirect fluorescent antibody technique. The enzyme was first detected in midpachytene primary spermatocytes and appeared to increase in concentration as spermatogenesis progressed to the spermatid. LDH-X synthesis, therefore, is initiated in the primary spermatocyte and continues at least until sometime during spermiogenesis. The uniform fluorescence of the cells containing LDH-X suggests that it is distributed throughout the cytoplasm rather than restricted to organelles such as mitochondria.

Original languageEnglish (US)
Pages (from-to)375-384
Number of pages10
JournalDevelopmental Biology
Volume57
Issue number2
DOIs
StatePublished - Jun 1977

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ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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