TY - JOUR
T1 - Immunohistochemical localization of LDH-X during spermatogenesis in mouse testes
AU - Hintz, Marie
AU - Goldberg, Erwin
N1 - Funding Information:
We gratefully acknowledge intellectual and technical assistance from Dr. T. Wheat, Dr. J. Lerum, L. Lauterbach, P. Larramendi, and P. Bentley. This work was supported in part by NIH Grant No. ROl HD05863, Biomedical Sciences Support Grant No. FR7-28-05, and Contract No. 73085 with the World Health Organization.
PY - 1977/6
Y1 - 1977/6
N2 - Testis-specific lactate dehydrogenase, LDH-X, was localized spatially and temporally in the germinal epithelium of the mouse by the indirect fluorescent antibody technique. The enzyme was first detected in midpachytene primary spermatocytes and appeared to increase in concentration as spermatogenesis progressed to the spermatid. LDH-X synthesis, therefore, is initiated in the primary spermatocyte and continues at least until sometime during spermiogenesis. The uniform fluorescence of the cells containing LDH-X suggests that it is distributed throughout the cytoplasm rather than restricted to organelles such as mitochondria.
AB - Testis-specific lactate dehydrogenase, LDH-X, was localized spatially and temporally in the germinal epithelium of the mouse by the indirect fluorescent antibody technique. The enzyme was first detected in midpachytene primary spermatocytes and appeared to increase in concentration as spermatogenesis progressed to the spermatid. LDH-X synthesis, therefore, is initiated in the primary spermatocyte and continues at least until sometime during spermiogenesis. The uniform fluorescence of the cells containing LDH-X suggests that it is distributed throughout the cytoplasm rather than restricted to organelles such as mitochondria.
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U2 - 10.1016/0012-1606(77)90222-6
DO - 10.1016/0012-1606(77)90222-6
M3 - Article
C2 - 326599
AN - SCOPUS:0017385461
SN - 0012-1606
VL - 57
SP - 375
EP - 384
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -