Immunohistochemical localization of pancreatic exocrine enzymes in normal and neoplastic pancreatic acinar epithelium of rat

L. J. Hansen, M. Mangkornkanok/Mark, J. K. Reddy

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10 Scopus citations

Abstract

The transplantable pancreatic acinar carcinomas of rat established recently provide useful model systems to examine the compositon of secretory proteins as well as the secretory process in transformed pancreatic exocrine epithelium. The neoplastic acinar cells exhibit considerable variation in the extent of cytodifferentiation. In the present study the enzymatic profile of this heterogeneous tumor cell population has been investigated by the indirect immunofluorescent technique using antibodies against six pancreatic enzymes. By immunofluorescence, all neoplastic cells stained positively for the six enzymes tested: amylase, lipase, carboxypeptidase A, chymotrypsinogen, trypsinogen, and ribonuclease. Some variability in the intensity of immunofluorescence was noted, suggesting possible quantitative differences in the content of a given enzyme among tumor cells. These observations suggest that neoplastic acinar cells with or without secretory granules contain secretory proteins, but to a variable extent.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume29
Issue number2
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Anatomy
  • Histology

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