Immunohistochemical localization of phosphoglycerate kinase isozymes in mouse testes

John L. Vandeberg*, Chi‐Yu ‐Y Lee, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We have used specific antibodies against mouse phosphoglycerate kinase (PGK) isozymes in an immunohistochemical technique to determine which cells in testicular cross sections contain PGK‐A and which contain PGK‐B. Phosphoglycerate kinase‐A is highly concentrated in interstitial cells and is weakly expressed in Sertoli cells and throughout the germinal epithelium. Phosphoglycerate kinase‐B is highly concentrated in spermatids and spermatozoa, but it is not detectable in stage 8 spermatids or earlier gametogenic stages, or in somatic cells of the testis. These results confirm that PGK‐B is a sperm‐specific isozyme in mice.

Original languageEnglish (US)
Pages (from-to)435-441
Number of pages7
JournalJournal of Experimental Zoology
Volume217
Issue number3
DOIs
StatePublished - Sep 1981

ASJC Scopus subject areas

  • Animal Science and Zoology

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