Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells

Do Sik Min, Myung Jong Kim, Hyun Kyung Jeong, Young Han Lee, Hyun Kim, Hee Sup Shin, Sung Ho Ryu, Pann Ghill Suh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We have developed a monoclonal antibody, β4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-β4 (PLC-β4). Tissue distribution of 130 kDa PLC-β4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-β4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-β4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-β4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-β4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)9-12
Number of pages4
JournalNeuroscience Letters
Volume292
Issue number1
DOIs
StatePublished - Sep 29 2000

Keywords

  • Immunohistochemistry
  • Monoclonal antibody
  • Phosphoinositide-specific phospholipase C
  • Phospholipase C-β4
  • Purkinje cell

ASJC Scopus subject areas

  • Neuroscience(all)

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