Immunological comparison of biotinidase in serum from normal and biotinidase-deficient individuals

Barry Wolf; James B. Miller; Jeanne Hymes; Julie Secor McVoy; Yuka Ishikawa; Emmanuel Shapira

doi:10.1016/0009-8981(87)90104-5

Immunological comparison of biotinidase in serum from normal and biotinidase-deficient individuals

Barry Wolf^*, James B. Miller, Jeanne Hymes, Julie Secor McVoy, Yuka Ishikawa, Emmanuel Shapira

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

An antiserum specific to enzymatically active human serum biotinidase was prepared. Using this antiserum, two immunologically cross-reacting protein fractions, only one of which corresponds to the active enzyme, were observed in sera from individuals with normal biotinidase activity. Neither of these protein fractions was detected in sera from 18 individuals with biotinidase deficiency from 15 families.

Original language	English (US)
Pages (from-to)	27-32
Number of pages	6
Journal	Clinica Chimica Acta
Volume	164
Issue number	1
DOIs	https://doi.org/10.1016/0009-8981(87)90104-5
State	Published - Apr 15 1987

Keywords

Biotin
Biotinidase
Biotinidase deficiency
Immunological studies

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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AU - Miller, James B.

AU - Hymes, Jeanne

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AU - Ishikawa, Yuka

AU - Shapira, Emmanuel

PY - 1987/4/15

Y1 - 1987/4/15

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AB - An antiserum specific to enzymatically active human serum biotinidase was prepared. Using this antiserum, two immunologically cross-reacting protein fractions, only one of which corresponds to the active enzyme, were observed in sera from individuals with normal biotinidase activity. Neither of these protein fractions was detected in sera from 18 individuals with biotinidase deficiency from 15 families.

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KW - Biotinidase

KW - Biotinidase deficiency

KW - Immunological studies

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Immunological comparison of biotinidase in serum from normal and biotinidase-deficient individuals

Abstract

Keywords

ASJC Scopus subject areas

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