Immunological comparison of biotinidase in serum from normal and biotinidase-deficient individuals

Barry Wolf*, James B. Miller, Jeanne Hymes, Julie Secor McVoy, Yuka Ishikawa, Emmanuel Shapira

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

An antiserum specific to enzymatically active human serum biotinidase was prepared. Using this antiserum, two immunologically cross-reacting protein fractions, only one of which corresponds to the active enzyme, were observed in sera from individuals with normal biotinidase activity. Neither of these protein fractions was detected in sera from 18 individuals with biotinidase deficiency from 15 families.

Original languageEnglish (US)
Pages (from-to)27-32
Number of pages6
JournalClinica Chimica Acta
Volume164
Issue number1
DOIs
StatePublished - Apr 15 1987

Keywords

  • Biotin
  • Biotinidase
  • Biotinidase deficiency
  • Immunological studies

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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