Abstract
An antiserum specific to enzymatically active human serum biotinidase was prepared. Using this antiserum, two immunologically cross-reacting protein fractions, only one of which corresponds to the active enzyme, were observed in sera from individuals with normal biotinidase activity. Neither of these protein fractions was detected in sera from 18 individuals with biotinidase deficiency from 15 families.
Original language | English (US) |
---|---|
Pages (from-to) | 27-32 |
Number of pages | 6 |
Journal | Clinica Chimica Acta |
Volume | 164 |
Issue number | 1 |
DOIs | |
State | Published - Apr 15 1987 |
Funding
This work was supportedb y grantsf rom the National Institute of Health (AM 25675a nd AM 33022)a nd from the National Foundation - March of Dimes (6-342).
Keywords
- Biotin
- Biotinidase
- Biotinidase deficiency
- Immunological studies
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Biochemistry, medical