Cryostatic sections of the rat large bowel tumors induced by 1,2-dimethylhydrazine were stained with monoclonal antibodies against different proteins of intermediate filaments: (a) against prekeratin (mol. mass 49,000, PK49) found in many epithelial cells and (b) against vimentin, a constituent of intermediate filaments of mesenchymal cells. Immunofluorescence study showed that large bowel tumor cells as well as normal cells of this organ contain PK49 but not vimentin. The high sensitivity of the method allowed one to clearly identify small invasive nodules and groups of tumor cells not visible in the usual histologic preparations. Moreover, in some cases single atypical tumor cells were identified in tumor stroma and in the submucosal layer underlying the tumor, that were indistinguishable from normal mesenchymal cells at the light microscopy level.
|Number of pages
|Byulleten Eksperimentalnoi Biologii i Meditsiny
|Published - 1985
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)