Immunosuppressive factor(s) specific for L‐glutamic acid50‐L‐tyrosine50 IV. In vitro activity and immunochemical properties

J. Thèze, Carl Waltenbaugh, R. N. Germain, B. Benacerraf*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The responsiveness of BALB/c spleen cells to the terpolymer L‐glutamic acid60‐L‐alanine30‐L‐tyrosine10(GAT), the copolymer of L‐glutamic acid50‐L‐tyrosine50 (GT) and the corresponding complexes with methylated bovine serum albumin (MBSA) has been studied in vitro in a modified Mishell‐Dutton culture system, Cultures of BALB/c spleen cells respond to GAT, GAT‐MBSA and GT‐MBSA over a wide dose range. Contrary to in vivo findings BALB/c spleen cell cultures respond to GT in vitro although in a much narrower dose range. Lymphoid extracts from GT‐primed BALB/c or B10.BR mice (GT‐TSF) specifically suppress the GT‐MBS A response in vitro. This culture system has allowed us to further investigate immunochemical properties of both BALB/c and B10.BR GT‐TSF. GT‐TSF from both strains display affinity for GT‐Sepharose and bear determinants encoded by the I region of the H‐2 complex. Moreover, we demonstrate that the suppressive activity from B10.BR mice is a molecule or a molecular complex which displays affinity for GT and bears determinants of the I‐J subregion of the H‐2k haplotype.

Original languageEnglish (US)
Pages (from-to)705-710
Number of pages6
JournalEuropean Journal of Immunology
Issue number10
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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