Immunotherapy in acute arsenic poisoning

Jerrold B. Leikin*, Robin E. Goldman-Leikin, Michael A. Evans, Stanley Wiener, Daniel O. Hryhorczuk

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We investigated the use of immunotherapy on the treatment of sodium arsenite toxicity. Female balb/c mice injected with arsanilic acid conjugated to a carrier protein (ovalbumin) were shown to produce antibodies (arsenic reactive serum, ARS) reactive with arsanilic acid and sodium arsenite. Serum was tested for anti-ARS antibodies using a solid phase radioimmunoassay. The antisera bound to ARS conjugated to the synthetic copolymer glutamic acid60 tyrosine30 when diluted as high as 1:4096. Following multiple injections of 100 μg of arsanilic acid - ovalbumin compound, mortality on injection with sodium arsenite 0.87 mg/kg i.p. one week later decreased to 0 deaths in 22 pretreated mice vs 9 deaths in 29 untreated mice (31% mortality; p < 005). No decrease in mortality was noted at higher challenges (1.15 mg/kg) of sodium arsenite. Antisera from pretreated mice was injected 0.1 cc i.p. into 12 week old female balb/c mice followed by an injection of sodium arsenite 0.87 mg/kg i.p. at 10 minutes. Again a protective effect was observed with 0 deaths in 18 mice vs eight deaths in 21 mice (38%; p < 005). Seventeen additional mice were given an injection of 0.87 mg/kg i.p. of sodium arsenite. After 30 minutes, all mice became symptomatic whereupon antisera 0.1 cc i.p. was given. The one day mortality (2/17, 12% was possibly lower than the combined control mortality (17/50, 34%; p < 0.07). There was no change in mortality noted when antisera was administered to mice acutely exposed to 5 mg/kg HgCl2.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalClinical Toxicology
Volume29
Issue number1
DOIs
StatePublished - Jan 1 1991

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Keywords

  • Arsanilic antibody
  • Arsenic poisoning
  • Arsenite
  • Immunotherapy
  • Mouse
  • Sodium

ASJC Scopus subject areas

  • Toxicology

Cite this

Leikin, J. B., Goldman-Leikin, R. E., Evans, M. A., Wiener, S., & Hryhorczuk, D. O. (1991). Immunotherapy in acute arsenic poisoning. Clinical Toxicology, 29(1), 59-70. https://doi.org/10.3109/15563659109038598