Impact of treadmill locomotor training on skeletal muscle IGF1 and myogenic regulatory factors in spinal cord injured rats

Min Liu, Jennifer E. Stevens-Lapsley, Arun Jayaraman, Fan Ye, Christine Conover, Glenn A. Walter, Prodip Bose, Floyd J. Thompson, Stephen E. Borst, Krista Vandenborne*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The objective of this study was to determine the impact of treadmill locomotor training on the expression of insulin-like growth factor I (IGF1) and changes in myogenic regulatory factors (MRFs) in rat soleus muscle following spinal cord injury (SCI). Moderate, midthoracic (T8) contusion SCIs were produced using a NYU (New York University) impactor. Animals were randomly assigned to treadmill training or untrained groups. Rats in the training group were trained starting at 1 week after SCI, for either 3 bouts of 20 min over 1.5 days or 10 bouts over 5 days. Five days of treadmill training completely prevented the decrease in soleus Wber size resulting from SCI. In addition, treadmill training triggered increases in IGF1, MGF and IGFBP4 mRNA expression, and a concurrent reduction of IGFBP5 mRNA in skeletal muscle. Locomotor training also caused an increase in markers of muscle regeneration, including small muscle Wbers expressing embryonic myosin and Pax7 positive nuclei and increased expression of the MRFs, myogenin and MyoD. We concluded that treadmill locomotor training ameliorated muscle atrophy in moderate contusion SCI rats. Traininginduced muscle regeneration and Wber hypertrophy following SCI was associated with an increase in IGF1, an increase in Pax7 positive nuclei, and upregulation of MRFs.

Original languageEnglish (US)
Pages (from-to)709-720
Number of pages12
JournalEuropean Journal of Applied Physiology
Issue number4
StatePublished - Jul 2010


  • IGF1
  • Locomotor training
  • Muscle regeneration
  • Skeletal muscle
  • Spinal cord injury

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)


Dive into the research topics of 'Impact of treadmill locomotor training on skeletal muscle IGF1 and myogenic regulatory factors in spinal cord injured rats'. Together they form a unique fingerprint.

Cite this