Impacts of air pollution, temperature, and relative humidity on leukocyte distribution: An epigenetic perspective

Xu Gao*, Elena Colicino, Jincheng Shen, Marianthi Anna Kioumourtzoglou, Allan C. Just, Jamaji C. Nwanaji-Enwerem, Brent Coull, Xihong Lin, Pantel Vokonas, Yinan Zheng, Lifang Hou, Joel Schwartz, Andrea A. Baccarelli

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Exploring the associations of air pollution and weather variables with blood leukocyte distribution is critical to understand the impacts of environmental exposures on the human immune system. Objectives: As previous analyses have been mainly based on data from cell counters, which might not be feasible in epidemiologic studies including large populations of long-stored blood samples, we aimed to expand the understanding of this topic by employing the leukocyte distribution estimated by DNA methylation profiles. Methods: We measured DNA methylation profiles in blood samples using Illumina HumanMethylation450 BeadChip from 1519 visits of 774 Caucasian males participating in the Normative Aging Study. Leukocyte distribution was estimated using Houseman's and Horvath's algorithms. Data on air pollution exposure, temperature, and relative humidity within 28 days before each blood draw was obtained. Results: After fully adjusting for potential covariates, PM2.5, black carbon, particle number, carbon monoxide, nitrogen dioxide, sulfur dioxide, temperature, and relative humidity were associated with the proportions of at least one subtype of leukocytes. Particularly, an interquartile range-higher 28-day average exposure of PM2.5 was associated with 0.147-, 0.054- and 0.101-unit lower proportions (z-scored) of plasma cells, naïve CD8+ T cells, and natural killers, respectively, and 0.059- and 0.161-unit higher proportions (z-scored) of naïve CD4+ T cells and CD8+ T cells, respectively. Conclusions: Our study suggests that short-term air pollution exposure, temperature, and relative humidity are associated with leukocyte distribution. Our study further provides a successful attempt to use epigenetic patterns to assess the influences of environmental exposures on human immune profiles.

Original languageEnglish (US)
Pages (from-to)395-405
Number of pages11
JournalEnvironment International
Volume126
DOIs
StatePublished - May 2019

Fingerprint

relative humidity
atmospheric pollution
blood
pollution exposure
methylation
temperature
DNA
nitrogen dioxide
immune system
black carbon
sulfur dioxide
carbon monoxide
weather
plasma
distribution
exposure

Keywords

  • Air pollution
  • DNA methylation
  • Environmental health
  • Epigenetic epidemiology
  • Leukocyte distribution
  • Weather variations

ASJC Scopus subject areas

  • Environmental Science(all)

Cite this

Gao, X., Colicino, E., Shen, J., Kioumourtzoglou, M. A., Just, A. C., Nwanaji-Enwerem, J. C., ... Baccarelli, A. A. (2019). Impacts of air pollution, temperature, and relative humidity on leukocyte distribution: An epigenetic perspective. Environment International, 126, 395-405. https://doi.org/10.1016/j.envint.2019.02.053
Gao, Xu ; Colicino, Elena ; Shen, Jincheng ; Kioumourtzoglou, Marianthi Anna ; Just, Allan C. ; Nwanaji-Enwerem, Jamaji C. ; Coull, Brent ; Lin, Xihong ; Vokonas, Pantel ; Zheng, Yinan ; Hou, Lifang ; Schwartz, Joel ; Baccarelli, Andrea A. / Impacts of air pollution, temperature, and relative humidity on leukocyte distribution : An epigenetic perspective. In: Environment International. 2019 ; Vol. 126. pp. 395-405.
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abstract = "Background: Exploring the associations of air pollution and weather variables with blood leukocyte distribution is critical to understand the impacts of environmental exposures on the human immune system. Objectives: As previous analyses have been mainly based on data from cell counters, which might not be feasible in epidemiologic studies including large populations of long-stored blood samples, we aimed to expand the understanding of this topic by employing the leukocyte distribution estimated by DNA methylation profiles. Methods: We measured DNA methylation profiles in blood samples using Illumina HumanMethylation450 BeadChip from 1519 visits of 774 Caucasian males participating in the Normative Aging Study. Leukocyte distribution was estimated using Houseman's and Horvath's algorithms. Data on air pollution exposure, temperature, and relative humidity within 28 days before each blood draw was obtained. Results: After fully adjusting for potential covariates, PM2.5, black carbon, particle number, carbon monoxide, nitrogen dioxide, sulfur dioxide, temperature, and relative humidity were associated with the proportions of at least one subtype of leukocytes. Particularly, an interquartile range-higher 28-day average exposure of PM2.5 was associated with 0.147-, 0.054- and 0.101-unit lower proportions (z-scored) of plasma cells, na{\"i}ve CD8+ T cells, and natural killers, respectively, and 0.059- and 0.161-unit higher proportions (z-scored) of na{\"i}ve CD4+ T cells and CD8+ T cells, respectively. Conclusions: Our study suggests that short-term air pollution exposure, temperature, and relative humidity are associated with leukocyte distribution. Our study further provides a successful attempt to use epigenetic patterns to assess the influences of environmental exposures on human immune profiles.",
keywords = "Air pollution, DNA methylation, Environmental health, Epigenetic epidemiology, Leukocyte distribution, Weather variations",
author = "Xu Gao and Elena Colicino and Jincheng Shen and Kioumourtzoglou, {Marianthi Anna} and Just, {Allan C.} and Nwanaji-Enwerem, {Jamaji C.} and Brent Coull and Xihong Lin and Pantel Vokonas and Yinan Zheng and Lifang Hou and Joel Schwartz and Baccarelli, {Andrea A.}",
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Gao, X, Colicino, E, Shen, J, Kioumourtzoglou, MA, Just, AC, Nwanaji-Enwerem, JC, Coull, B, Lin, X, Vokonas, P, Zheng, Y, Hou, L, Schwartz, J & Baccarelli, AA 2019, 'Impacts of air pollution, temperature, and relative humidity on leukocyte distribution: An epigenetic perspective', Environment International, vol. 126, pp. 395-405. https://doi.org/10.1016/j.envint.2019.02.053

Impacts of air pollution, temperature, and relative humidity on leukocyte distribution : An epigenetic perspective. / Gao, Xu; Colicino, Elena; Shen, Jincheng; Kioumourtzoglou, Marianthi Anna; Just, Allan C.; Nwanaji-Enwerem, Jamaji C.; Coull, Brent; Lin, Xihong; Vokonas, Pantel; Zheng, Yinan; Hou, Lifang; Schwartz, Joel; Baccarelli, Andrea A.

In: Environment International, Vol. 126, 05.2019, p. 395-405.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impacts of air pollution, temperature, and relative humidity on leukocyte distribution

T2 - An epigenetic perspective

AU - Gao, Xu

AU - Colicino, Elena

AU - Shen, Jincheng

AU - Kioumourtzoglou, Marianthi Anna

AU - Just, Allan C.

AU - Nwanaji-Enwerem, Jamaji C.

AU - Coull, Brent

AU - Lin, Xihong

AU - Vokonas, Pantel

AU - Zheng, Yinan

AU - Hou, Lifang

AU - Schwartz, Joel

AU - Baccarelli, Andrea A.

PY - 2019/5

Y1 - 2019/5

N2 - Background: Exploring the associations of air pollution and weather variables with blood leukocyte distribution is critical to understand the impacts of environmental exposures on the human immune system. Objectives: As previous analyses have been mainly based on data from cell counters, which might not be feasible in epidemiologic studies including large populations of long-stored blood samples, we aimed to expand the understanding of this topic by employing the leukocyte distribution estimated by DNA methylation profiles. Methods: We measured DNA methylation profiles in blood samples using Illumina HumanMethylation450 BeadChip from 1519 visits of 774 Caucasian males participating in the Normative Aging Study. Leukocyte distribution was estimated using Houseman's and Horvath's algorithms. Data on air pollution exposure, temperature, and relative humidity within 28 days before each blood draw was obtained. Results: After fully adjusting for potential covariates, PM2.5, black carbon, particle number, carbon monoxide, nitrogen dioxide, sulfur dioxide, temperature, and relative humidity were associated with the proportions of at least one subtype of leukocytes. Particularly, an interquartile range-higher 28-day average exposure of PM2.5 was associated with 0.147-, 0.054- and 0.101-unit lower proportions (z-scored) of plasma cells, naïve CD8+ T cells, and natural killers, respectively, and 0.059- and 0.161-unit higher proportions (z-scored) of naïve CD4+ T cells and CD8+ T cells, respectively. Conclusions: Our study suggests that short-term air pollution exposure, temperature, and relative humidity are associated with leukocyte distribution. Our study further provides a successful attempt to use epigenetic patterns to assess the influences of environmental exposures on human immune profiles.

AB - Background: Exploring the associations of air pollution and weather variables with blood leukocyte distribution is critical to understand the impacts of environmental exposures on the human immune system. Objectives: As previous analyses have been mainly based on data from cell counters, which might not be feasible in epidemiologic studies including large populations of long-stored blood samples, we aimed to expand the understanding of this topic by employing the leukocyte distribution estimated by DNA methylation profiles. Methods: We measured DNA methylation profiles in blood samples using Illumina HumanMethylation450 BeadChip from 1519 visits of 774 Caucasian males participating in the Normative Aging Study. Leukocyte distribution was estimated using Houseman's and Horvath's algorithms. Data on air pollution exposure, temperature, and relative humidity within 28 days before each blood draw was obtained. Results: After fully adjusting for potential covariates, PM2.5, black carbon, particle number, carbon monoxide, nitrogen dioxide, sulfur dioxide, temperature, and relative humidity were associated with the proportions of at least one subtype of leukocytes. Particularly, an interquartile range-higher 28-day average exposure of PM2.5 was associated with 0.147-, 0.054- and 0.101-unit lower proportions (z-scored) of plasma cells, naïve CD8+ T cells, and natural killers, respectively, and 0.059- and 0.161-unit higher proportions (z-scored) of naïve CD4+ T cells and CD8+ T cells, respectively. Conclusions: Our study suggests that short-term air pollution exposure, temperature, and relative humidity are associated with leukocyte distribution. Our study further provides a successful attempt to use epigenetic patterns to assess the influences of environmental exposures on human immune profiles.

KW - Air pollution

KW - DNA methylation

KW - Environmental health

KW - Epigenetic epidemiology

KW - Leukocyte distribution

KW - Weather variations

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