Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis

Guo Zhong Tao, Bo Liu, Rong Zhang, Gigi Liu, Fizan Abdullah, Mary Cay Harris, Mary L. Brandt, Richard A. Ehrenkranz, Corinna Bowers, Camilia R. Martin, R. Lawrence Moss, Karl G. Sylvester*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Necrotizing enterocolitis (NEC) is the most common gastrointestinal (GI) medical/surgical emergency of the newborn and a leading cause of preterm neonate morbidity and mortality. NEC is a challenge to diagnose since it often shares similar clinical features with neonatal sepsis. In the present study, plasma protein profiling was compared among NEC, sepsis and control cohorts using gel electrophoresis, immunoblot and mass spectrometry. We observed significant impairment in the formation of fibrinogen-3 dimers (FGG-dimer) in the plasma of newborns with NEC that could efficiently differentiate NEC and sepsis with a high level of sensitivity and specificity. Interestingly, the impaired FGG-dimer formation could be restored in NEC plasma by the addition of exogenous active factor XIII (FXIII). Enzymatic activity of FXIII was determined to be significantly lower in NEC subject plasma for crosslinking FGG when compared to sepsis. These findings demonstrate a potential novel biomarker and related biologic mechanism for diagnosing NEC, as well as suggest a possible therapeutic strategy.

Original languageEnglish (US)
Article number13119
JournalScientific reports
Volume5
DOIs
StatePublished - Aug 17 2015

ASJC Scopus subject areas

  • General

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