Abstract
Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex-/-;RAG1-/- chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220-CD19+ cells in the bone marrow. Hex-/-;RAG1-/- chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220-CD19+ cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 556-561 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 100 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 21 2003 |
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Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex. / Bogue, Clifford W.; Zhang, Ping Xia; McGrath, James; Jacobs, Harris C.; Fuleihan, Ramsay L.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 2, 21.01.2003, p. 556-561.Research output: Contribution to journal › Article
TY - JOUR
T1 - Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex
AU - Bogue, Clifford W.
AU - Zhang, Ping Xia
AU - McGrath, James
AU - Jacobs, Harris C.
AU - Fuleihan, Ramsay L.
PY - 2003/1/21
Y1 - 2003/1/21
N2 - Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex-/-;RAG1-/- chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220-CD19+ cells in the bone marrow. Hex-/-;RAG1-/- chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220-CD19+ cells.
AB - Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex-/-;RAG1-/- chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220-CD19+ cells in the bone marrow. Hex-/-;RAG1-/- chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220-CD19+ cells.
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U2 - 10.1073/pnas.0236979100
DO - 10.1073/pnas.0236979100
M3 - Article
VL - 100
SP - 556
EP - 561
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 2
ER -