Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex

Clifford W. Bogue*, Ping Xia Zhang, James McGrath, Harris C. Jacobs, Ramsay L. Fuleihan

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex-/-;RAG1-/- chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220-CD19+ cells in the bone marrow. Hex-/-;RAG1-/- chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220-CD19+ cells.

Original languageEnglish (US)
Pages (from-to)556-561
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number2
DOIs
StatePublished - Jan 21 2003

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Homeobox Genes
B-Lymphocytes
T Independent Antigens
T-Lymphocytes
B-Lymphoid Precursor Cells
Blastocyst
Plasma Cells
Bone Marrow Cells
Antibody Formation
Immunoglobulin M
Immunoglobulin G
Lymphocytes
Antigens
Antibodies
Serum

ASJC Scopus subject areas

  • General

Cite this

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title = "Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex",
abstract = "Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex-/-;RAG1-/- chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220-CD19+ cells in the bone marrow. Hex-/-;RAG1-/- chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220-CD19+ cells.",
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Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex. / Bogue, Clifford W.; Zhang, Ping Xia; McGrath, James; Jacobs, Harris C.; Fuleihan, Ramsay L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 2, 21.01.2003, p. 556-561.

Research output: Contribution to journalArticle

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AB - Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex-/-;RAG1-/- chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220-CD19+ cells in the bone marrow. Hex-/-;RAG1-/- chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220-CD19+ cells.

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