Impaired T cell function in RANTES-deficient mice

Yasuhiko Makino*, Donald N. Cook, Oliver Smithies, Olivia Y. Hwang, Eric G. Neilson, Laurence A. Turka, Hiroshi Sato, Andrew D. Wells, Theodore M. Danoff

*Corresponding author for this work

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

The chemokine RANTES is a chemoattractant for monocytes and T cells and is postulated to participate in many aspects of the immune response. To evaluate the biological roles of RANTES in vivo, we generated RANTES-deficient (-/-) mice and characterized their T cell function. In cutaneous delayed-type hypersensitivity assays, a 50% reduction in ear and footpad swelling was seen in -/- mice compared to +/+ mice. In vitro, polyclonal and antigen-specific T cell proliferation was decreased. Quantitative analysis using the fluorescent dye carboxy-fluorescein succinimidyl ester revealed that this proliferative defect was due both to fewer antigen-reactive T cells and to a reduction in the capacity of these cells to proliferate. In addition, IFN-γ and IL-2 production by the -/- T cells was dramatically decreased. Together, these data suggest that RANTES is required for normal T cell functions as well as for recruiting monocytes and T cells to sites of inflammation.

Original languageEnglish (US)
Pages (from-to)302-309
Number of pages8
JournalClinical Immunology
Volume102
Issue number3
DOIs
StatePublished - 2002

Keywords

  • Cellular proliferation
  • Chemokines
  • Delayed-type hypersensitivity
  • Knockout
  • Th1/Th2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Makino, Y., Cook, D. N., Smithies, O., Hwang, O. Y., Neilson, E. G., Turka, L. A., Sato, H., Wells, A. D., & Danoff, T. M. (2002). Impaired T cell function in RANTES-deficient mice. Clinical Immunology, 102(3), 302-309. https://doi.org/10.1006/clim.2001.5178