Impedance characterization and modelling of an improved patch clamp device

Y. Zhao, S. Inayat, D. A. Dikin, R. S. Ruoff, J. B. Troy

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The gold standard for studying the properties of ion channels is the patch clamp, a technique that measures pA currents passing through ion channels in an isolated patch of cell membrane. The authors sought to design an improved device for whole-cell patch clamp recordings using nanoscale pipettes which more readily form seals with the cell membrane and a movable metal electrode to decrease the access resistance, increase the signal bandwidth, and clear debris from the pipette tip. Electrical characterization of the instrument by impedance spectroscopy showed that advancing the metal electrode towards the pipette tip decreased the impedance distinctly in different frequency bands, the greatest decrease occurring between 1 and 100kHz, and the smallest at > 100kHz. Finite element simulations of the electrode, incorporating the electrode/electrolyte interface, suggest that optimal signal transmission can be achieved with a thick-walled pipette, nearly filled with a large Ag/AgCl electrode, whose impedance can be reduced through surface modification.

Original languageEnglish (US)
Pages (from-to)121-131
Number of pages11
JournalProceedings of the Institution of Mechanical Engineers, Part N: Journal of Nanoengineering and Nanosystems
Volume223
Issue number3
DOIs
StatePublished - Jan 1 2010

Keywords

  • electrode/electrolyte interface
  • electrophysiology
  • finite element modelling
  • movable electrode
  • neuroscience
  • patch clamp

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Impedance characterization and modelling of an improved patch clamp device'. Together they form a unique fingerprint.

Cite this