Abstract
Dysregulated inflammation underlies many human diseases, and measures of responsiveness to activation, and sensitivity to inhibition, provide important information beyond baseline assessments of chronic inflammation. This study implements a simplified cell culture protocol in a school-based setting, using finger stick capillary blood collected from 333 adolescents (age 11.4–15.6 years) incubated with lipopolysaccharide (LPS). Median cytokine responses for IL6, IL1β, and TNFα were 61.9, 26.2, and 11.2 pg/mL, respectively. Samples were also incubated with LPS and glucocorticoid (GC) to measure GC sensitivity. Median responses were reduced in the presence of GC inhibition for IL6 (20.3 pg/mL), IL1β (10.5 pg/mL), and TNFα (3.3 pg/mL). Minimally invasive cell culture protocols provide novel opportunities for measuring inflammatory phenotypes in a wide range of non-clinical settings.
| Original language | English (US) |
|---|---|
| Article number | e24077 |
| Journal | American Journal of Human Biology |
| Volume | 36 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2024 |
Funding
This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Programme (Grant agreement No. 853517) and from the Special Research Fund (BOF) of Ghent University (BOF Starting Grant, project number BOF/STA/202002/016). The project was also supported by funding from the Canadian Institute for Advanced Research (CIFAR).
ASJC Scopus subject areas
- Anatomy
- Ecology, Evolution, Behavior and Systematics
- Anthropology
- Genetics