Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice

Jianke Ren, Long Wang, Guoxiang Liu, Wen Zhang, Zhejin Sheng, Zhugang Wang, Jian Fei*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Recombinant fusion protein is widely used as an antigen to raise antibodies against the epitope of a target protein. However, the concomitant anticarrier antibody in resulting antiserum reduces the production of the desired antibody and brings about unwanted non-specific immune reactions. It is proposed that the carrier protein transgenic animal could be used to solve this problem. To validate this hypothesis, enhanced green fluorescent protein (EGFP) transgenic mice were produced. By immunizing the mice with fusion protein His6HAtag-EGFP, we showed that the antiserum from the transgenic mice had higher titer antibody against His6HA tag and lower titer antibody against EGFP compared with that from wild-type mice. Therefore, this report describes an improved method to raise high titer antipeptide polyclonal antibody using EGFP transgenic mice that could have application potential in antibody preparation.

Original languageEnglish (US)
Pages (from-to)111-115
Number of pages5
JournalActa biochimica et biophysica Sinica
Volume40
Issue number2
DOIs
StatePublished - Feb 2008

Keywords

  • EGFP
  • Polyclonal antibody
  • Transgenic mice

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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