Improved rapid method for the isolation, purification and identification of collagen glycosides

Hector Garza; Nelson Bennett; Gladys P. Rodriguez

doi:10.1016/0021-9673(95)01296-6

Improved rapid method for the isolation, purification and identification of collagen glycosides

Hector Garza^*, Nelson Bennett, Gladys P. Rodriguez

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPLC) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.

Original language	English (US)
Pages (from-to)	385-389
Number of pages	5
Journal	Journal of Chromatography A
Volume	732
Issue number	2
DOIs	https://doi.org/10.1016/0021-9673(95)01296-6
State	Published - May 3 1996

Keywords

Amino acids
Collagen
Galactosyl hydroxylysine
Glucosylgalactosyl hydroxylysine
Glycosides
Hydroxylysine
Saccharides

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0021-9673(95)01296-6

Cite this

@article{ec997418d92342ef961da2eb3f4643b5,

title = "Improved rapid method for the isolation, purification and identification of collagen glycosides",

abstract = "An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPLC) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.",

keywords = "Amino acids, Collagen, Galactosyl hydroxylysine, Glucosylgalactosyl hydroxylysine, Glycosides, Hydroxylysine, Saccharides",

author = "Hector Garza and Nelson Bennett and Rodriguez, {Gladys P.}",

year = "1996",

month = may,

day = "3",

doi = "10.1016/0021-9673(95)01296-6",

language = "English (US)",

volume = "732",

pages = "385--389",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Improved rapid method for the isolation, purification and identification of collagen glycosides

AU - Garza, Hector

AU - Bennett, Nelson

AU - Rodriguez, Gladys P.

PY - 1996/5/3

Y1 - 1996/5/3

N2 - An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPLC) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.

AB - An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPLC) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.

KW - Amino acids

KW - Collagen

KW - Galactosyl hydroxylysine

KW - Glucosylgalactosyl hydroxylysine

KW - Glycosides

KW - Hydroxylysine

KW - Saccharides

UR - http://www.scopus.com/inward/record.url?scp=0029873776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029873776&partnerID=8YFLogxK

U2 - 10.1016/0021-9673(95)01296-6

DO - 10.1016/0021-9673(95)01296-6

M3 - Article

C2 - 8653203

AN - SCOPUS:0029873776

SN - 0021-9673

VL - 732

SP - 385

EP - 389

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 2

ER -

Improved rapid method for the isolation, purification and identification of collagen glycosides

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this