Improved rapid method for the isolation, purification and identification of collagen glycosides

Hector Garza*, Nelson Bennett, Gladys P. Rodriguez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPLC) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.

Original languageEnglish (US)
Pages (from-to)385-389
Number of pages5
JournalJournal of Chromatography A
Volume732
Issue number2
DOIs
StatePublished - May 3 1996

Keywords

  • Amino acids
  • Collagen
  • Galactosyl hydroxylysine
  • Glucosylgalactosyl hydroxylysine
  • Glycosides
  • Hydroxylysine
  • Saccharides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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