Improved sensitivity for solid-support invasive cleavage reactions with flow cytometry analysis

P. Wilkins Stevens*, K. V.N. Rao, J. G. Hall, V. Lyamichev, B. P. Neri, D. M. Kelso

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A new configuration of the solid-support invasive cleavage reaction provides a small reaction-volume format for high-sensitivity discrimination of nucleic acid targets with single nucleotide differences. With target concentrations as low as 2 amol/assay, the solid-support invasive cleavage reaction clearly distinguishes single base mutations. Two oligonucleotides tethered to the solid support hybridize to the target nucleic acid, forming a tripartite substrate that can be recognized and cleaved by Cleavase

Original languageEnglish (US)
Pages (from-to)198-203
Number of pages6
JournalBioTechniques
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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    Wilkins Stevens, P., Rao, K. V. N., Hall, J. G., Lyamichev, V., Neri, B. P., & Kelso, D. M. (2003). Improved sensitivity for solid-support invasive cleavage reactions with flow cytometry analysis. BioTechniques, 34(1), 198-203. https://doi.org/10.2144/03341dd09