The determination of levelsof IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBVinfected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBVinfections, including those unaccompanied by a heterophil antibody response.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Infectious Diseases|
|State||Published - Oct 1982|
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases