Improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus

Ciro V. Sumaya*, Yasmin Ench, Richard M. Pope

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The determination of levelsof IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBVinfected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBVinfections, including those unaccompanied by a heterophil antibody response.

Original languageEnglish (US)
Pages (from-to)518-523
Number of pages6
JournalJournal of Infectious Diseases
Volume146
Issue number4
DOIs
StatePublished - Oct 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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