Improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus

Ciro V. Sumaya; Yasmin Ench; Richard M. Pope

doi:10.1093/infdis/146.4.518

Improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus

Ciro V. Sumaya^*, Yasmin Ench, Richard M. Pope

^*Corresponding author for this work

Medicine, Rheumatology Division

Research output: Contribution to journal › Article › peer-review

Abstract

The determination of levelsof IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBVinfected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBVinfections, including those unaccompanied by a heterophil antibody response.

Original language	English (US)
Pages (from-to)	518-523
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	146
Issue number	4
DOIs	https://doi.org/10.1093/infdis/146.4.518
State	Published - Oct 1982

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/146.4.518

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title = "Improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus",

abstract = "The determination of levelsof IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBVinfected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBVinfections, including those unaccompanied by a heterophil antibody response.",

author = "Sumaya, {Ciro V.} and Yasmin Ench and Pope, {Richard M.}",

note = "Funding Information: This study was supported in part by grants no. 15646 from the National Institute of Allergy and Infectious Diseases and no. 25686 from the National Cancer Institute.",

year = "1982",

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T1 - Improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus

AU - Sumaya, Ciro V.

AU - Ench, Yasmin

AU - Pope, Richard M.

N1 - Funding Information: This study was supported in part by grants no. 15646 from the National Institute of Allergy and Infectious Diseases and no. 25686 from the National Cancer Institute.

PY - 1982/10

Y1 - 1982/10

N2 - The determination of levelsof IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBVinfected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBVinfections, including those unaccompanied by a heterophil antibody response.

AB - The determination of levelsof IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBVinfected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBVinfections, including those unaccompanied by a heterophil antibody response.

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Improved test for igm antibody to epstein-barr virus using an absorption step with staphylococcus aureus

Abstract

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